轻工学报2025，Vol.40Issue(4)：96-107,12.DOI:10.12187/2025.04.011
果胶酶粗酶液与漆酶粗酶液复配酶解膨胀梗丝的工艺优化
Optimization of enzymatic hydrolysis process for expanded tobacco cut stem using combined crude enzymes of pectinase and laccase
摘要
Abstract
Pectinase-producing strain TS-1 and laccase-producing strain TS-2 were isolated from tobacco field soil and decayed tobacco stems.Morphological observation and molecular biological identification confirmed their taxo-nomic status as Aspergillus niger and Phanerodontia chrysosporium,respectively.Enzyme production conditions were optimized via single-factor experiments:for TS-1 pectinase production,the optimal parameters were tobacco stem powder concentration 50 g/L,fermentation temperature 30℃,and pH value 6.0;for TS-2 laccase produc-tion,the optimal conditions were tobacco stem powder concentration 50 g/L,(NH4)2SO4 concentration 0.6 g/L,and fermentation temperature 30℃.The hydrolysis conditions for pectin in expanded tobacco cut stems were opti-mized as crude pectinase dosage 240 U/g,hydrolysis time 4.0 h,liquid-to-solid ratio 1∶2,at 30℃;for lignin hydrolysis,the optimal conditions were crude laccase dosage 2.0 U/g,hydrolysis time 3.5 h,liquid-to-solid ratio 1∶2,at 30℃.The co-application of crude enzymes achieved optimal hydrolysis at pectinase dosage 170 U/g,laccase dosage 1.5 U/g,and hydrolysis time 3.0 h.Post-hydrolysis,the sensory evaluation score of expanded cut stems increased by 8.2 points compared to untreated samples,accompanied by a significant elevation in aroma con-stituent content.关键词
烟梗/果胶酶/漆酶/酶解工艺/膨胀梗丝/感官品质/致香成分Key words
tobacco stem/pectinase/laccase/enzymatic hydrolysis process/expanded tobacco cut stem/sensory quality/aroma constituent分类
轻工纺织引用本文复制引用
齐汉如,欧阳少丰,李玉,杨雪鹏,赵建国..果胶酶粗酶液与漆酶粗酶液复配酶解膨胀梗丝的工艺优化[J].轻工学报,2025,40(4):96-107,12.基金项目
中国烟草总公司重点研发专项项目(110202102020) （110202102020）
河南省科技攻关项目(242102320122) （242102320122）
