中国食品添加剂2025，Vol.36Issue(8)：45-53,9.DOI:10.19804/j.issn1006-2513.2025.8.007
基于UPLC-Q-TOF-MS/MS技术鉴定韭菜子中的苦味物质及其免疫调节活性研究
Identification of bitter substances from Allii tuberosi semen based on UPLC-Q-TOF-MS/MS and their immunoregulatory activity
摘要
Abstract
In order to clarify the sources of bitter components in Allii tuberosi semen,column separation and chromatographic separation techniques were used to isolate,purify bitter substances from allii tuberosi semen extract.Then,ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)combined with sensory evaluation was used to identify the obtained bitter components from Allii tuberosi semen.The results showed that the bitter substances in Allii tuberosi semen were mainly thymine,guanosine,and trigonelline.Compared to the Allii tuberosi semen extract obtained by ethanol extraction,the bitter values of the Allii tuberosi semen extract obtained by aqueous extraction were higher.The zebrafish model was used to evaluate the immunomodulatory activity of bitter components from Allii tuberosi semen extract.The bitter component trigonelline in Allii tuberosi semen extract demonstrated significant enhancement of macrophage phagocytosis and alleviation of T-cell depletion in zebrafish at low and medium doses.The research results provide data support for targeted bitterness reduction in Allii tuberosi semen extract while preserving their immunomodulatory activity.关键词
韭菜子/提取溶剂/超高效液相色谱-串联四级杆飞行时间质谱/苦味/斑马鱼/免疫调节Key words
Allii tuberosi semen/extract solvent/ultra performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)/bitterness/zebrafish/immunomodulation分类
轻工纺织引用本文复制引用
陈阳,赖富丽,郝桂芳,陈丽,唐万,王喆,杨强,徐剑..基于UPLC-Q-TOF-MS/MS技术鉴定韭菜子中的苦味物质及其免疫调节活性研究[J].中国食品添加剂,2025,36(8):45-53,9.基金项目
湖北省科技厅自然科学基金联合基金项目(2022CFD076) （2022CFD076）
