食品与机械2025，Vol.41Issue(8)：61-65,5.DOI:10.13652/j.spjx.1003.5788.2024.80939
Sin-QuEChERS Nano-UPLC-MS/MS检测大米中甲氨基阿维菌素苯甲酸盐残留
Determination of residual emamectin benzoate in rice by Sin-QuEChERS Nano-UPLC-MS/MS
摘要
Abstract
[Objective]To establish a new Sin-QuEChERS nano-triple quadrupole liquid chromatography-mass spectrometry(UPLC-MS/MS)method for detecting emamectin benzoate residues in rice.[Methods]After extraction with 0.1%formic acid-acetonitrile,the sample is purified with N-primary secondary amine(PSA)and multi-walled carbon nanotube(MWCNTs)fillers.The mobile phase is a gradient elution of 0.1%formic acid water and acetonitrile.Separation is performed using a C18 chromatographic column,and the detection is carried out using a triple quadrupole liquid chromatography-tandem mass spectrometry system.[Results]The quantification limit of emamectin benzoate is 0.001 5 mg/kg,and the detection limit of the method is 0.000 5 mg/kg.A good linear relationship is observed for emamectin benzoate in the concentration range of 1～100 ng/mL,with a correlation coefficient greater than 0.999 5.At 2,10,and 50 µg/kg spiking levels,the average recovery rate of emamectin benzoate is 86.9%～110.2%,with a relative standard deviation of 0.98%～7.21%.[Conclusion]This method is efficient,simple,stable,and highly sensitive,making it suitable for detecting emamectin benzoate in rice.关键词
Sin-QuEChERS Nano/甲维盐/大米Key words
Sin-QuEChERS Nano/emamectin benzoate/rice引用本文复制引用
罗宽能,黄子杰,黄朋勉,刘勇,杨姝萍,张洪刚..Sin-QuEChERS Nano-UPLC-MS/MS检测大米中甲氨基阿维菌素苯甲酸盐残留[J].食品与机械,2025,41(8):61-65,5.基金项目
怀化市科技计划创新项目(编号:2021R3120) （编号:2021R3120）
