食品与发酵工业2025，Vol.51Issue(17)：108-117,10.DOI:10.13995/j.cnki.11-1802/ts.040958
植物乳植杆菌KSFY01对氧化偶氮甲烷/硫酸葡聚糖钠诱导小鼠炎症相关性结直肠癌的效果及机制研究
Effects and mechanisms of Lactiplantibacillus plantarum KSFY01 on azoxymethane/dextran sodium sulfate-induced inflammation-associated colorectal cancer in mice
摘要
Abstract
A mouse model of inflammation-related colorectal cancer was established through the induction of azoxymethane(AOM)and dextran sodium sulfate(DSS)to investigate the effects and mechanisms of Lactiplantibacillus plantarum KSFY01(LP-KSFY01),iso-lated from traditional fermented yogurt in Xinjiang.The experimental design included a normal group,a model group,an aspirin group,as well as groups treated with low(1× 108 CFU/kg)and high(1× 109 CFU/kg)concentrations of LP-KSFY01.Except for the normal group,all mice underwent treatment with AOM/DSS.The aspirin group received daily oral doses of 67 mg/kg of aspirin,while the LP-KS-FY01 groups were administered daily oral suspensions of LP-KSFY01.Measurements were taken of body weight,organ weight,colon length,and the number of intestinal tumors,along with histopathological analyses of the rectum.Enzyme-linked immunosorbent assay was utilized to assess the levels of inflammatory cytokines in serum and colon tissues,and real-time fluorescence quantitative PCR was em-ployed to measure the mRNA expression of NF-κB and apoptosis-related factors in the colon.Results indicated that LP-KSFY01 interven-tion significantly mitigated weight loss and normalized organ indices,reduced the incidence of intestinal tumors,and improved histopatho-logical damage in the rectum.Furthermore,a decrease in the levels of inflammatory cytokines such as IL-6,IL-1β,TNF-α,and iNOS was observed in both serum and colon tissues(P＜0.05).The mRNA expression of IκBβ,p65,p50,p52,Bcl-2,and Bcl-xL was downregu-lated,while the expression of Bid and caspase-8 was upregulated(P＜0.05).Notably,a greater effect was noted with the high concentra-tion of LP-KSFY01 compared to the low concentration.In conclusion,LP-KSFY01 may play a role in alleviating intestinal inflammation progression,intervening in colorectal tumor development through modulating the NF-κB and apoptosis pathways,indicating a potential reg-ulatory effect on inflammation-related colorectal cancer in mice and suggesting further research value.关键词
植物乳植杆菌/氧化偶氮甲烷/硫酸葡聚糖钠/结直肠癌/NF-κB/细胞凋亡Key words
Lactiplantibacillus plantarum/azoxymethane(AOM)/dextran sodium sulfate(DSS)/inflammation-associated colorectal canc-er/NF-κB/apoptosis引用本文复制引用
余婷,赵欣,胡甜甜..植物乳植杆菌KSFY01对氧化偶氮甲烷/硫酸葡聚糖钠诱导小鼠炎症相关性结直肠癌的效果及机制研究[J].食品与发酵工业,2025,51(17):108-117,10.基金项目
重庆市教育委员会科学技术研究计划青年项目资助项目(KJQN202201601) （KJQN202201601）
重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0424) （cstc2021jcyj-msxmX0424）
