摘要
Abstract
To study the effects of perilla and mulberry leaf and honeysuckle solid beverage on immune function,an immunosup-pressed mouse model that was constructed using cyclophosphamide was investigated,mice were randomly divided into blank control group,model group and low(833 mg/kg·BW),medium(1 667 mg/kg·BW)and high(5 000 mg/kg·BW)dose solid beverage groups,immunological indices were measured.The results showed that the thymus index and spleen index of high dose group significantly in-creased;The number of RBC and HGB significantly increased in each dose group,while the number of PLT and HCT in the high-dose group significantly increased;The levels of TNF-α,INF-γ,and IL-1[3 in serum of high dose group were significantly downregulated.To study the antimutagenic effects of solid beverages,the Ames test,mouse bone marrow erythrocyte micronucleus test,and mouse spermato-cyte chromosome aberration test were selected.The results showed that solid beverage had an antagonistic effect on 2-aminofluorene-in-duced back mutation of Ames strain,the micronucleus rate of bone marrow cells significantly decreased in each dose group,the micronu-cleus inhibition rate of female mice in low,medium and high dose groups were 30.4％,46.4％,and 60.5％,respectively;the micronu-cleus inhibition rate of male mice in low,medium and high dose groups were 30.5％,46.9％,and 59.1％,respectively;the chromosome aberration rate of testicular spermatocyte in medium and high dose groups decreased significantly,and the inhibition rate in low,medium and high dose groups was 8.9％,25.9％,and 65.2％,respectively.In conclusion,perilla mulberry leaf and honeysuckle solid beverage have immunomodulatory and antimutagenic effects on mice.关键词
紫苏提取物/固体饮料/环磷酰胺/免疫功能/抗突变Key words
perilla extract/solid beverage/cyclophosphamide/immune function/antimutation
