食品与发酵工业2025，Vol.51Issue(17)：296-304,344,中插18-中插19,12.DOI:10.13995/j.cnki.11-1802/ts.042853
新疆樱桃李果皮花色苷的提取、纯化及成分分析
Extraction,purification and composition analysis of anthocyanins from Xinjiang Prunus cerasifera Ehrh.peel
摘要
Abstract
To enhance the resource utilization of anthocyanins from the peel of Xinjiang Prunus cerasifera Ehrh.,an environmentally friendly production process has been developed.This process utilized the fruit peel as raw material to efficiently produce high-purity antho-cyanins.Using the total anthocyanin content as the evaluation index,pectinase-assisted extraction was employed to optimize the extraction process through single-factor and orthogonal experiments.Macroporous adsorbent resin combined with polyamide resin was used for the pur-ification of anthocyanins,and ultra-high-performance liquid chromatography with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF/MS)was employed for composition analysis.The results showed that the optimal extraction conditions were as follows:pectinase dos-age of 0.2％,sample pH of 2.0,solid-to-liquid ratio of 1∶80(g∶mL),temperature of 70 ℃,and extraction time of 30 minutes.Under these conditions,the total anthocyanin content in the peel reached 1 718.35 mg/100 g.The optimal purification conditions using polyam-ide resin were as follows:sample pH of 3.0,sample volume of 10 BV,ethanol eluent concentration of 50％,elution volume of 3 BV,and flow rate of 2.5 mL/min.After purification,the total anthocyanin content reached 50.72％.UPLC-Q-TOF/MS analysis identified 30 com-ponents,including 9 anthocyanins,10 flavonoids,4 phenolic acids,and 7 phenylpropanoids.This study developed an integrated'pecti-nase-assisted extraction and dual-resin purification'system,which achieved green extraction and enrichment of anthocyanins.The combi-nation of chemical composition analysis of P.cerasifera peel with this innovative methodology provides a scientific foundation for both sus-tainable resource utilization and identification of pharmacologically active constituents.关键词
樱桃李/花色苷/提取/纯化/成分分析Key words
Prunus cerasifera Ehrh./anthocyanins/extraction/purification/composition analysis引用本文复制引用
谭峻杰,田占萍,西尔扎提·艾海提,吉舒仪,摆雅文,沈静,姚军..新疆樱桃李果皮花色苷的提取、纯化及成分分析[J].食品与发酵工业,2025,51(17):296-304,344,中插18-中插19,12.基金项目
国家自然科学基金项目(82360827) （82360827）
中央引导地方科技发展专项资金项目(ZYYD2023B11) （ZYYD2023B11）
新疆医科大学研究生创新创业项目(CXCY2025022) （CXCY2025022）
