中外葡萄与葡萄酒Issue(5)：10-20,11.DOI:10.13414/j.cnki.zwpp.2025.05.002
葡萄Ⅲ类过氧化物酶基因家族鉴定及表达分析
Identification and Expression Analysis of Grape Class Ⅲ Peroxidase Gene Family
摘要
Abstract
Class Ⅲ peroxidases(PRXs)play crucial roles in plant growth,development,and stress responses.In this study,74 VvPRXs genes were identified from the grape genome through genome-wide analysis.Phylogenetic classification divided them into five distinct groups.Domain and conserved motif analyses revealed that VvPRXs harbor the typical peroxidase domain,with highly conserved protein sequences.Collinearity analysis identified 17 tandem duplication and 11 segment duplication events in grape chromosomes,along with 39 and 34 orthologous gene pairs with Arabidopsis and rice,respectively.Cis-acting element analysis suggested potential roles of VvPRXs in growth regulation and hormone/stress signaling pathways.Expression profiling demonstrated that VvPRX36 acted as a central regulator in salt stress response,VvPRX16 and VvPRX46 were specifically responsive to cold stress,while VvPRX52,VvPRX51,VvPRX34,VvPRX68,VvPRX31,and VvPRX71 were markedly induced under drought conditions.Pathogen infection assays showed that Botrytis cinerea induced the upregulation of VvPRX71 and VvPRX33,whereas Xylella fastidiosa infection specifically activated VvPRX23 expression.These findings systematically elucidate the evolutionary features and stress-responsive mechanisms of the VvPRXs family,providing critical candidate genes for molecular breeding to enhance stress tolerance in grapevines.关键词
葡萄/Ⅲ类过氧化物酶/基因家族/胁迫响应Key words
grape/Class Ⅲ peroxidase/gene family/stress response分类
农业科技引用本文复制引用
朱昱枫,张渊博,王现行..葡萄Ⅲ类过氧化物酶基因家族鉴定及表达分析[J].中外葡萄与葡萄酒,2025,(5):10-20,11.基金项目
国家自然科学基金(32202423) （32202423）
国家自然科学基金(32372661) （32372661）
