食品科学2025，Vol.46Issue(19)：10-17,8.DOI:10.7506/spkx1002-6630-20250224-119
超声联合紫外处理对大肠杆菌O157:H7的杀菌作用及机制
Bactericidal Effect and Mechanism of Ultrasound Combined with Ultraviolet Treatment against Escherichia coli O157:H7
摘要
Abstract
This study aimed to investigate the synergistic bactericidal effect and mechanism of ultrasound combined with ultraviolet against Escherichia coli O157:H7.The inactivation kinetics was modeled,ultrastructural changes in bacterial cells were examined as well as alterations in the properties of the cell membrane,and oxidative stress levels were determined.The results revealed that after 40 s of combined treatment,the bacterial count was reduced by 8.3(lg(CFU/mL)).Significant impairment of cell morphology and leakage of cellular contents were observed.The combined treatment induced a cellular stress response within a short period of time,characterized by increased cellular esterase activity,ATP content and levels of reactive oxygen species(ROS)along with DNA damage.In conclusion,combined treatment with ultrasound and ultraviolet synergistically exerted a significant bactericidal effect.Ultrasound-induced cavitation enhances structural damage to cells,enabling ultraviolet to directly act on DNA.Moreover,both treatments work together to exacerbate oxidative stress,thereby interfering with cellular metabolism and exerting a synergistic bactericidal effect.关键词
超声/紫外/大肠杆菌O157:H7/杀菌机制/协同效应Key words
ultrasound/ultraviolet/Escherichia coli O157:H7/bactericidal mechanism/synergistic effect分类
轻工纺织引用本文复制引用
陈怡,吴雨豪,周建伟,刘东红,吕瑞玲..超声联合紫外处理对大肠杆菌O157:H7的杀菌作用及机制[J].食品科学,2025,46(19):10-17,8.基金项目
宁波市企业创新联合体项目(2022H006) （2022H006）
宁波市公益性科技计划项目(2022S139) （2022S139）
