食品科学2025，Vol.46Issue(19)：37-46,10.DOI:10.7506/spkx1002-6630-20250220-087
熊果酸改善高糖诱导的足细胞线粒体功能障碍的作用机制
Mechanism of Action of Ursolic Acid in Ameliorating Mitochondrial Dysfunction in Podocytes Induced by High Glucose
摘要
Abstract
Objective:This study aimed to investigate the protective effects of ursolic acid on mitochondrial dysfunction in podocytes induced by high glucose and to explore the underlying mechanism.Methods:The CCK-8 method was used to determine the experimental conditions and the experiments were divided into five groups:control(CN),high glucose(200 mmol/L,HG),low-dose ursolic acid(200 mmol/L glucose+0.703 μmol/L ursolic acid),medium-dose ursolic acid(200 mmol/L glucose+1.406 μmol/L ursolic acid)and high-dose ursolic acid(200 mmol/L glucose+2.813 μmol/L ursolic acid).The immunofluorescence method was used to observe mitochondrial morphology and detect the reactive oxygen species(ROS)level,the degree of mitochondrial permeability transition pore(mPTP)opening and mitochondrial membrane potential(MMP).Flow cytometry was used to detect the ROS level and the rate of apoptosis of podocytes.Western Blot was used to detect the expression of cyclophilin D(Cyp D),voltage-dependent anion channel(VDAC),Bcl-2 associated X protein(Bax),B-cell lymphoma-2(Bcl-2)and cytochrome c(Cyt c)proteins.Results:Compared with the CN group,the proliferation rate of podocytes was significantly reduced by intervention of 200 mmol/L glucose(P<0.01),which was therefore used for modeling.Compared with the HG group,all doses of ursolic acid significantly increased the proliferation rate of podocytes(P<0.01).Immunofluorescence assays indicated that they restored mitochondrial morphology,inhibited ROS overproduction,repressed the abnormal opening of mPTP and increased MMP.The results of flow cytometry showed that ursolic acid at all three doses reduced the apoptosis rate of podocytes compared with the HG group(P<0.01).The Western Blot results showed that compared with the HG group,they significantly down-regulated the expression of Cyp D,VDAC,Bax and Cyt c proteins but up-regulated the expression of Bcl-2 protein.Conclusion:Ursolic acid may exert its effect against diabetic nephropathy by inhibiting mitochondrial dysfunction and apoptosis caused by HG-induced oxidative stress in podocytes.关键词
熊果酸/糖尿病肾病/线粒体功能障碍/细胞凋亡Key words
ursolic acid/diabetic nephropathy/mitochondrial dysfunction/apoptosis分类
医药卫生引用本文复制引用
顾惠贤,阿依夏木·麦提托合提,吴思宇,蒋祥龙,姚蓝..熊果酸改善高糖诱导的足细胞线粒体功能障碍的作用机制[J].食品科学,2025,46(19):37-46,10.基金项目
新疆维吾尔自治区天山英才计划第三期(2021241) （2021241）
