食品科学2025，Vol.46Issue(19)：57-67,11.DOI:10.7506/spkx1002-6630-20250430-255
亚油酸调控大豆7S和11S球蛋白互作机制及其对蛋白膜理化性质的影响
Mechanism by Which Linoleic Acid Regulates the Interaction between Soybean 7S and 11S Globulins and Its Effects on the Physicochemical Properties of Protein Films
摘要
Abstract
To elucidate the regulatory effect of linoleic acid(LA)on the interaction between β-conglycinin(7S)and glycinin(11S)and the physicochemical properties of their composite films,the interactions of LA with the 7S-11S complex and glycerol were investigated using molecular dynamics(MD)simulation,and the effect of LA on the performance of 7S-11S composite films was examine.MD simulation demonstrated that the radius of gyration,solvent-accessible surface area,and hydrophobic surface area of the 7S-11S complex increased with increasing LA content,while intermolecular interactions weakened,with the total binding free energy increasing from-114.31 to-1.61 kcal/mol.Meanwhile,hydrogen bonds and van der Waals forces between LA and the complex as well as those between LA and glycerol strengthened with increasing LA content.The addition of LA reduced the tensile strength and water vapor permeability of 7S-11S composite films but enhanced the elongation at break and surface water contact angle.Fourier transform infrared spectroscopy(FTIR)analysis indicated that LA weakened the O-H stretching vibration peak while intensifying the C-H stretching vibrations at 3 011,2 926,and 2 855 cm-1.Moreover,the proportion of hydrophobic interactions in the 7S-11S composite films increased with LA content,leading to the formation of insoluble aggregates of high-molecular-mass components involved in hydrogen bonding and hydrophobic interactions.This study has elucidated that LA modulates protein-based film extensibility and hydrophobicity by regulating 7S-11S interactions and intermolecular forces,providing theoretical insights for developing soy protein-based edible films.关键词
分子动力学模拟/大豆球蛋白/亚油酸/相互作用机制/蛋白膜/疏水性Key words
molecular dynamics simulation/soybean globulin/linoleic acid/interaction mechanism/protein films/hydrophobicity分类
轻工纺织引用本文复制引用
刘美铃,石林凡,任中阳,翁武银..亚油酸调控大豆7S和11S球蛋白互作机制及其对蛋白膜理化性质的影响[J].食品科学,2025,46(19):57-67,11.基金项目
国家自然科学基金面上项目(32272266) （32272266）
