食品科学2025，Vol.46Issue(19)：79-88,10.DOI:10.7506/spkx1002-6630-20250421-167
B-大麦醇溶蛋白的分离优化与黏附效果
Isolation Optimization and Mucoadhesion Analysis of B-Hordein
摘要
Abstract
This study characterized the selective complex coacervation between hordein and pectin to confirm the selective binding behavior of different hordein components in order to develop a low-cost,readily scalable protein isolation method.Besides,the isolation of B-hordein was optimized and the adhesion properties of different components were also evaluated.The results demonstrated that B-hordein,the major component of hordein,preferentially bound to two types of pectin to form coacervates.High-methoxyl pectin(HMP)exhibited weaker binding affinity to B-hordein,as evidenced by the fact that the supernatant of the complex system contained a significant amount of B-hordein.In contrast,low-methoxyl pectin(LMP)showed stronger binding affinity to B-hordein.Protein spectrum analysis revealed that high-purity B-hordein could be obtained by collecting the coacervates from the Hordein/LMP1:1 system.Adjusting the pH enabled the secondary separation of B-hordein from LMP in the coacervates,with a B-hordein recovery of 65.29%.Meanwhile,B-hordein-based composites exhibited the best adhesion performance.This study provides a theoretical foundation for promoting the development of protein isolation technologies.关键词
B-大麦醇溶蛋白/果胶/选择性结合行为/组分分离/黏附Key words
B-hordein/pectin/selective binding behavior/component isolation/mucoadhesion分类
轻工纺织引用本文复制引用
李非凡,迟晓君,秦洋,卜令金,时宇航..B-大麦醇溶蛋白的分离优化与黏附效果[J].食品科学,2025,46(19):79-88,10.基金项目
山东省现代农业产业技术体系中草药体系淄博综合试验站(鲁农科教字[2024]19号) （鲁农科教字[2024]19号）
新疆自治区重点研发计划项目(2024B04030) （2024B04030）
山东农业工程学院高层次人才科研启动经费资助项目(2024GCCZR-19) （2024GCCZR-19）
