中国食品添加剂2025，Vol.36Issue(9)：43-50,8.DOI:10.19804/j.issn1006-2513.2025.9.006
黑果腺肋花楸不同部位总多酚、总黄酮提取工艺优化及其抗氧化活性研究
Optimization of extraction process of total polyphenols and total flavonoids from different parts of black chokeberry and their antioxidant activities
摘要
Abstract
Black chokeberry was used as the raw material,and total polyphenols and total flavonoids contents in its different parts(roots,stems,leaves,fruits)were measured by Folin-Ciocalteu colorimetric method and sodium nitrite-aluminum nitrate method,respectively.Extraction procedures of total polyphenols and total flavonoids from black chokeberry were optimized by orthogonal experiments.Antioxidant activities of different parts of black chokeberry were evaluated by DPPH and ABTS radical scavenging assays,hydroxyl radical scavenging rate,and reducing power assays.The results showed that total polyphenol content varied significantly among different parts of black chokeberry,ranked as leaves＞fruits＞stems＞roots;total flavonoid content among different parts of black chokeberry also differed significantly,ranked as fruits＞leaves＞stems＞roots.The optimal extraction conditions for total polyphenols were determined as extraction with 55%solution containing 0.2%hydrochloric acid at a material-to-liquid ratio of 1:25,ultrasonically extracted at 40℃for 65 minutes;for total flavonoids,extraction with 60%ethanol at a material-to-liquid ratio of 1:40,ultrasonically extracted at 65℃for 20 minutes was optimized.The optimized extraction methods for total polyphenols and flavonoids were found to be stable and feasible.All parts of black chokeberry exhibited strong antioxidant activities,with leaves and fruits showing higher effects comparable to vitamin C,while stems and roots showed lower activities than fruits.关键词
黑果腺肋花楸/总多酚/总黄酮/提取工艺/抗氧化活性Key words
black chokeberry/total polyphenols/total flavonoids/extraction process/antioxidant activity分类
轻工纺织引用本文复制引用
苗卫东,周新宇,吴俊英,丛龙娇,王琪瑶,史锐..黑果腺肋花楸不同部位总多酚、总黄酮提取工艺优化及其抗氧化活性研究[J].中国食品添加剂,2025,36(9):43-50,8.基金项目
2024年辽宁省教育厅高校基本科研项目-重点项目(LJ212410162035):桑属不同种桑枝的显微特征指数及抗炎和降糖活性研究 （LJ212410162035）
