肉类研究2025，Vol.39Issue(12)：69-75,7.DOI:10.7506/rlyj1001-8123-20250223-054
改良QuEChERS-超高效液相色谱-串联质谱法快速测定猪肉中12种利尿剂残留量
Rapid Determination of 12 Diuretic Residues in Pork by Modified QuEChERS Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method for the simultaneous determination of 12 diuretic residues in pork was developed using a modified QuEChERS approach and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).After homogenization,the sample was extracted with acetonitrile and cleaned up with MgSO4,C18 and primary secondary amine.Separation was performed on an Eclipse Plus C18 column(100 mm×2.1 mm,1.8 μm)using a mobile phase consisting of 3 mmol/L ammonium formate-0.1%formic acid in water and acetonitrile.Detection was carried out using UPLC-MS/MS in the multiple reaction monitoring(MRM)mode.All analytes exhibited good linearity over the concentration range evaluated with correlation coefficients(R2)>0.99.The limits of quantitation(LOQ)were 2-5 μg/kg.The average recoveries at three spiked levels of LOQ,2 LOQ and 10 LOQ ranged from 85.8%to 112.0%,with relative standard deviations(RSDs)of 0.5%-5.1%.The proposed method is a simple yet efficient,sensitive and selective approach for the rapid detection of the 12 diuretics in pork.关键词
QuEChERS/超高效液相色谱-串联质谱法/利尿剂/猪肉Key words
QuEChERS/ultra-high performance liquid chromatography-tandem mass spectrometry/diuretics/pork分类
化学化工引用本文复制引用
占昊,余晓峰,宣磊,孔维恒,刘雯,陈冉冉,蒙晓笛,周晓莹,吕亚宁,韩芳..改良QuEChERS-超高效液相色谱-串联质谱法快速测定猪肉中12种利尿剂残留量[J].肉类研究,2025,39(12):69-75,7.基金项目
"十四五"国家重点研发计划重点专项(2023YFF0611501) （2023YFF0611501）
国产质谱类设备海关验证评价技术和体系研究项目(2023HK141) （2023HK141）
