食品工业科技2025，Vol.46Issue(19)：54-65,12.DOI:10.13386/j.issn1002-0306.2024070104
玫瑰茄花色苷及其酯化产物结构表征、稳定性及体外降血糖活性
Structural Characterization,Stability and Hypoglycemic Activity in Vitro of Roselle Anthocyanins and Its Esterified Product
摘要
Abstract
To investigate the structure,stability properties and hypoglycemic activity in vitro of roselle anthocyanins and its esterification product,anthocyanins were modified by esterification with succinic anhydride.The anthocyanins with and without esterification were characterized by UV spectroscopy(UV-Vis),infrared spectroscopy(FTIR),X-ray diffraction(XRD),thermogravimetric analysis(TGA)and differential scanning calorimetry(DSC).The anthocyanins content,color value,color and stability were determined and compared.The inhibitory effects and types of anthocyanins on α-amylase and α-glucosidase before and after esterification were explored.The results showed that after esterification,the maximum ultraviolet absorption wavelength of anthocyanins was blue-shifted,ester carbonyl was increased,the structure became more loosely and the thermal stability decreased.In addition,the content and color value of anthocyanins reduced after esterification,thus the solution color became lighter,but the stability under light,high temperature and different pH conditions of esterified anthocyanins improved.At 37 ℃ and pH6.8 for 15 min,the anthocyanins and its esterification product could effectively inhibit the activities of two enzymes.The two kinds of anthocyanins both demonstrated a mixed-type inhibition on α-amylase and α-glucosidase with IC50 values of 4.507,3.708 mg/mL and 4.045,2.950 mg/mL,respectively.Furthermore,the esterification product had higher enzyme inhibitory activities.Anyway,after esterification,the molecular structure,color and its stability properties of roselle anthocyanins were changed,which obviously enhanced its hypoglycemic activity in vitro.关键词
玫瑰茄/花色苷/酯化/结构表征/稳定性/降血糖活性Key words
roselle/anthocyanin/esterification/structural characterization/stability/hypoglycemic activity分类
轻工纺织引用本文复制引用
王丽霞,吴浈浈,周三女..玫瑰茄花色苷及其酯化产物结构表征、稳定性及体外降血糖活性[J].食品工业科技,2025,46(19):54-65,12.基金项目
莆田学院科研项目(2019022) （2019022）
国家级大学生创新训练项目(202111498011,202011498013) （202111498011,202011498013）
莆田市科技局项目(2022NZ2001ptxy18). （2022NZ2001ptxy18）
