食品工业科技2025，Vol.46Issue(19)：75-84,10.DOI:10.13386/j.issn1002-0306.2024090294
介质阻挡放电等离子体辅助糖基化对β-乳球蛋白抗氧化活性的影响及构效关系研究
Effect of Dielectric Barrier Discharge Plasma-assisted Glycation on the Antioxidant Activity of β-Lactoglobulin and Its Structure-Activity Relationship
摘要
Abstract
This study examined the antioxidant activity of β-lactoglobulin(β-LG)and its hydrolysate after the treatment of dielectric barrier discharge(DBD)plasma-assisted glycation.The relationship between the structure and antioxidant activity of β-LG was analyzed by measuring the internal fluorescence,ultraviolet absorption spectrum,sulfhydryl content,surface hydrophobicity,average particle size,and secondary structure of treated β-LG.The results showed that after DBD plasma-assisted glycation for 5 min,the DPPH radical scavenging capacity of β-LG reached 129.8 μmol trolox equivalent(TE)/g,and the iron reduction capacity was increased to 75.1 μmol Fe2+/g.The hydrolysate exhibited further enhancement in both DPPH radical scavenging capacity and iron reduction capacity,reaching 193.5 μmol TE/g and 95.5 μmol Fe2+/g,respectively.After DBD plasma-assisted glycation for 5 min,the endogenous fluorescence,ultraviolet absorption intensity,the relative content of α-helix,sulfhydryl group content and surface hydrophobicity(P＜0.05)of β-LG significantly decreased,whereas the average particle size and the relative content of β-fold increased.In conclusion,DBD plasma-assisted glycation can effectively improve the antioxidant activity of β-L G by changing its structure.关键词
介质阻挡放电等离子体/糖基化/β-乳球蛋白/抗氧化活性/分子结构Key words
dielectric barrier discharge plasma/glycation/β-lactoglobulin/antioxidant activity/molecular structure分类
轻工纺织引用本文复制引用
翟娅菲,王宇浩,汤国新,牛力源,张艳艳,相启森..介质阻挡放电等离子体辅助糖基化对β-乳球蛋白抗氧化活性的影响及构效关系研究[J].食品工业科技,2025,46(19):75-84,10.基金项目
国家自然科学基金青年科学基金项目(32102135) （32102135）
河南省科技攻关项目(242102110109). （242102110109）
