食品与发酵工业2025，Vol.51Issue(18)：127-135,9.DOI:10.13995/j.cnki.11-1802/ts.041619
酪蛋白胶束与甜菜红素相互作用及其复合物性质研究
Study on the interaction between micellar casein and betanin,and the properties of their complexes
摘要
Abstract
Betanin(BT)is a kind of water-soluble nitrogenous pigment with multiple biological activities,but its stability is low,which limits its application in the food industry.In this study,micellar casein(MC)was used as a matrix to prepare BT-MC complexes.The interaction between BT and MC was investigated,as well as the effects of heat treatment(40,60,80 ℃),pH(8.0,11.0),and addition of metal ions(Fe3+,Zn2+)on the stability of BT with or without MC loading.The results showed that BT combined with MC through van der Waals force and hydrogen bonds,the binding process of which was exothermic with the binding constant(Ka)of(8.18±6.17)× 103 L/mol at 298 K.After combination,the conformation of the MC was altered,in which the content of α-helix increased while the contents of β-turn and random coil reduced.Combining with MC significantly enhanced the retention rate of BT after heat treatment,with the retention rate of 1 mmol/L BT increasing from(29.22±1.64)％to(91.88±0.77)％after 2 hours of heating at 80 ℃,while 5 mmol/L BT increased from(23.90±1.11)％to(81.07±0.73)％.In addition,MC improved the stability of BT under alkaline con-ditions,with the retention rate of 1 mmol/L BT increasing from(47.62±0.33)％to(94.00±0.54)％after 2 hours at pH 11.0,while for 5 mmol/L BT increasing from(56.88±0.42)％to(95.54±0.17)％.However,the MC combination reduced the stability of BT in the presence of metal ions.Thus,combination with MC was beneficial to improve the thermal stability and pH stability of BT.MC could be used as a natural carrier for BT loading to expand its application.关键词
甜菜红素/酪蛋白胶束/相互作用/稳定性/保留率Key words
betanin/micellar casein/interaction/stability/retention rate引用本文复制引用
曾学而,柯发辉,杨敏,张文娟,魏彦明..酪蛋白胶束与甜菜红素相互作用及其复合物性质研究[J].食品与发酵工业,2025,51(18):127-135,9.基金项目
甘肃省重点研发计划项目(23YFNA0017) （23YFNA0017）
2024年创新驱动助力工程项目(GXH20240328-15) （GXH20240328-15）
兰州市人才创新创业项目(2023-RC-22) （2023-RC-22）
2023年省级制造业高质量发展专项 （）
