食品与发酵工业2025，Vol.51Issue(19)：238-247,中插4-中插5,12.DOI:10.13995/j.cnki.11-1802/ts.042967
基于纳米碳酸钙的牡蛎肽递送体系构建与结构表征及其消化特性研究
Construction,structural characterization and digestion characteristics of oyster peptides delivery system based on nano-calcium carbonate
摘要
Abstract
Oyster bioactive peptides(OBP)exhibit multiple biological activities including antioxidant,hypoglycemic,and hypolipi-demic effects.However,the poor stability of OBP in gastrointestinal environments significantly limits their efficacy.To enhance the stability of OBP and reduce the waste of oyster shell resources,this study first synthesized nano-calcium carbonate(CaCO3 NPs)from oyster shell.The CaCO3 NPs were then modified with sodium alginate(ALG),and the modification process was optimized.Subsequently,OBP was loaded onto ALG-CaCO3 NPs,followed by polyethylene glycol modification,to construct an OBP-CaCO3 NPs nano-system.Finally,the study investigated the structural stability,in vitro release,and digestive characteristics of the nano-system.The orthogonal experimental re-sults indicated that the optimal modification process parameters for CaCO3 NPs were:ALG concentration of 5 mg/mL,temperature of 35℃,and reaction time of 2 h.The nano-system showed an average particle size of(288.7±7.6)nm and zeta potential of(-30.5±0.8)mV,achieving encapsulation efficiency of(35.69±2.22)％and loading capacity of(19.71±1.60)％.Fourier transform infrared spectroscopy analysis revealed that ALG modified CaCO3 NPs by forming an'egg box'structure through the cross-linking of carboxyl group and Ca2+,while OBP were loaded inside the carrier through electrostatic effect.The results of in vitro release experiments showed that the cumulative release rate of the nano-system in the simulated gastric environment(pH 2.0)for 36 hours was only(40.31±0.63)％,while the release rate in the intestinal environment(pH 6.8)significantly increased to(93.12±3.76)％,indicating the nano-system had the characteristics of targeted intestinal slow release.Digestion stability experiments demonstrated that the nano-system could significantly improve the pancreatic lipase inhibitory activity of OBP,the inhibitory rate increased from(23.66±0.93)％to(54.73±1.37)％after gastric digestion,and from(4.75±0.38)％to(24.67±1.31)％after gastrointestinal digestion.This study provides theoretical support for the design of stable delivery systems for functional peptides,while offering a novel approach for the high-value utilization of oyster shell waste.关键词
牡蛎肽/纳米碳酸钙/海藻酸钠修饰/纳米递送系统/消化特性Key words
oyster peptides/nano-calcium carbonate/sodium alginate modification/nano delivery system/digestive stability引用本文复制引用
王婧,陈忠琴,张萌萌,张卓铅,曹文红,谭明堂,朱国萍,高加龙,林海生,郑惠娜..基于纳米碳酸钙的牡蛎肽递送体系构建与结构表征及其消化特性研究[J].食品与发酵工业,2025,51(19):238-247,中插4-中插5,12.基金项目
湛江市海洋青年人才创新项目(2021E05017) （2021E05017）
广东海洋大学科研启动费资助项目(060302042007) （060302042007）
国家自然科学基金项目(32201971) （32201971）
广东省基础与应用基础研究基金项目(2024A1515011763) （2024A1515011763）
广东省现代农业产业技术体系贝藻创新团队项目(2024CXTD23) （2024CXTD23）
