食品工业科技2025，Vol.46Issue(20)：31-40,10.DOI:10.13386/j.issn1002-0306.2024110216
功能性寡糖的硒化修饰及其体外抗氧化活性分析
Selenium Modification of Functional Oligosaccharides and Analysis of Their Antioxidant Activity in Vitro
摘要
Abstract
In this experiment,three functional oligosaccharides(glucoosaccharides,raffinose,trehalose)and sodium selenite were used as raw materials to prepare selenide oligosaccharides by HNO3-Na2SeO3 method.The structures of three functional oligosaccharides and their selenide oligosaccharides were characterized by UV and IR spectra.The effect of selenium modification on the antioxidant activity of oligosaccharides was studied by in vitro antioxidant activity test.The selenium contents of selenose-oligosaccharide,selenoraffinose and selenotrehalose prepared under the experimental conditions were 10.450,10.514 and 10.488 mg/g,respectively.The structural characterization results showed that the three selenide oligosaccharides all contained the characteristic peak of selenite Se=O,and the basic skeleton of the oligosaccharides was preserved,indicating that the three functional oligosaccharides were successfully selenized by HNO3-Na2SeO3 method.In vitro antioxidant activity experiment showed that after selenization modification,the DPPH free radical scavenging rate of glucooligosaccharide,raffinose and trehalose was slightly increased,while the hydroxyl free radical and ABTS cationic free radical scavenging effect of the three was significantly improved.When the sample concentration was 10 mg/mL,the hydroxyl radical scavenging rates of selenooligosaccharides,selenoraffinose and selenotrehalose were about 84.13％,98.17％and 98.17％,respectively,and the cation free radical scavenging rates of ABTS were about 40.47％,48.27％and 90.40％,respectively.The scavenging rates of hydroxyl free radicals and ABTS cationic free radicals of trehalose selenide were similar to that of VC at corresponding concentrations.In conclusion,the scavenging activity of hydroxyl radical and ABTS cationic radical of oligosaccharides can be significantly improved by selenization modification,which can provide reference for the development of selenium-supplementing preparations and functional foods related to selenide oligosaccharides.关键词
葡寡糖/棉子糖/海藻糖/硒化修饰/结构表征/体外抗氧化活性Key words
glucooligosaccharides/raffinose/trehalose/selenide modification/structural characterization/antioxidant activity in vitro分类
轻工纺织引用本文复制引用
王清,曹珍,孙鹤,雷磊,王腾达,朱静,李建芳,刘涛..功能性寡糖的硒化修饰及其体外抗氧化活性分析[J].食品工业科技,2025,46(20):31-40,10.基金项目
河南省科技攻关项目(232102230071,242102110090) （232102230071,242102110090）
河南省高等学校重点科研项目(23B550006,24B550017,24B180016) （23B550006,24B550017,24B180016）
信阳农林学院高水平科研孵化器建设基金(FCL202110) （FCL202110）
信阳农林学院青年骨干教师培养项目(2022年度王清). （2022年度王清）
