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基于UPLC-ESI-MS/MS探究低盐处理对两色金鸡菊叶片类黄酮代谢物的影响

江虹 李志元 郭凡荣 秦勇

中国食品添加剂2025,Vol.36Issue(10):42-50,9.
中国食品添加剂2025,Vol.36Issue(10):42-50,9.DOI:10.19804/j.issn1006-2513.2025.10.006

基于UPLC-ESI-MS/MS探究低盐处理对两色金鸡菊叶片类黄酮代谢物的影响

Effects of low salt treatment on flavonoid metabolites of Coreopsis tinctoria Nutt.leaves based on UPLC-ESI-MS/MS

江虹 1李志元 1郭凡荣 1秦勇1

作者信息

  • 1. 新疆农业大学园艺学院,乌鲁木齐 830052
  • 折叠

摘要

Abstract

Based on the ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-ESI-MS/MS)system,leaves flavonoid components and contents of Coreopsis tinctoria Nutt.(C.tinctoria)seedlings(4-6 leaves,30 d)treated with 50 mmol/L NaCl at 0(CK),12,24 and 48 h were compared and analyzed.The key flavonoid metabolites induced by the low salt treatment was identified.The results showed that a total of 136 flavonoids were identified,including 58 kinds of flavones,35 kinds of flavonols,8 kinds of chalcones,8 kinds of isoflavones,7 kinds of anthocyanins,7 kinds of dihydroflavone,6 kinds of dihydroflavonols,5 kinds of flavonoid C-glycosides and 2 kinds of orange flavonoids.Among the treatment groups,24 flavonoids(DAFs)were selected,and 7 of them were significantly enriched in the flavonoids synthesis pathway and its branches,which were naringenin,pinobanksin,2'-Hydroxygenistein,eriodictyol,dihydroquercetin(taxifolin),cyanidin-3-O-glucoside,and quercetin-3-O-rutinoside(rutin),indicating the important biological function of these flavonoids under low salt conditions.The results of this study can provide available theoretical basis on the stress resistance and quality improvement of C.tinctoria.

关键词

UPLC-ESI-MS/MS/两色金鸡菊/低盐胁迫/类黄酮代谢物

Key words

UPLC-ESI-MS/MS/Coreopsis tinctoria Nutt./low-salt stress/flavonoid metabolism

分类

轻工纺织

引用本文复制引用

江虹,李志元,郭凡荣,秦勇..基于UPLC-ESI-MS/MS探究低盐处理对两色金鸡菊叶片类黄酮代谢物的影响[J].中国食品添加剂,2025,36(10):42-50,9.

基金项目

新疆维吾尔自治区重点研发计划项目(2023B02010) (2023B02010)

新疆维吾尔自治区人才发展基金"天池英才"引进计划项目 ()

中国食品添加剂

1006-2513

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