轻工学报2025，Vol.40Issue(5)：29-36,8.DOI:10.12187/2025.05.004
基于高通量测序和纯培养联用技术的药曲微生物多样性解析
Microbial diversity analysis of medicinal koji based on the combined techniques of high-throughput sequencing and pure culture
摘要
Abstract
MiSeq high-throughput sequencing and pure culture techniques were combined to analyze the microbial diversity of medicinal koji samples collected from Nanchang city,Jiangxi province.The aim was to reveal the microbial community structure within the koji.The results showed that the richness and diversity of bacterial taxa in the medicinal koji were significantly higher than those of the fungi.The dominant bacterial genera were Pediococcus and Levilactobacillus,with average relative abundances of 38.04%and 13.00%,respectively.The dominant fungal genera were Candida and Saccharomycopsis,with average relative abundances of 78.30%and 17.92%,respectively.Nine strains of bacteria isolated from medicinal koji were identified as three species:Pediococcus pentosaceus,Pedi-ococcus acidilactici,and Bacillus licheniformis.Seven strains of fungi were identified as four species:Saccharomy-copsis fibuligera,Saccharomycopsis malanga,Pichia kudriavzevii,and Saccharomyces cerevisiae.关键词
药曲/MiSeq高通量测序/纯培养/微生物多样性Key words
medicinal koji/MiSeq high-throughput sequencing/pure culture/microbial diversity分类
轻工纺织引用本文复制引用
黄怡,蔡文超,余培荣,陈炜,单春会,郭壮,王玉荣..基于高通量测序和纯培养联用技术的药曲微生物多样性解析[J].轻工学报,2025,40(5):29-36,8.基金项目
新疆生产建设兵团第七师胡杨河市"揭榜挂帅"项目(QS2024011) （QS2024011）
湖北省高等学校优秀中青年科技创新团队计划项目(T2023022) （T2023022）
