轻工学报2025，Vol.40Issue(5)：44-54,11.DOI:10.12187/2025.05.006
一株高耐受粗甘油枯草芽孢杆菌的分子机制研究
Investigation into the molecular mechanism of a Bacillus subtilis strain exhibiting high tolerance to crude glycerol
摘要
Abstract
The Bacillus subtilis strain with high tolerance to crude glycerol was selected as the research subject,and its tolerance mechanism to high-concentration crude glycerol was investigated using whole genome and transcriptome sequencing technologies.The results showed that 23 mutantgenes were identified in the evolved strain,involving key pathways such as the ABC transport system,nicotinic acid and nicotinamide metabolism,quorum sensing,and spore formation.Compared to the parent strain,the evolved strain exhibited significantly upregulated gene expression in pathways related to glycerol(ester)metabolism,the tricarboxylic acid cycle,purine metabolism,the ABC transport system,and energy metabolism.The synthesis metabolism of fatty acids and biotin of the evolved strainwere markedly enhanced.Furthermore,the evolved strain partially lost its spore-forming capacity,with spore formation rates decreasing by 38.0%and 52.3%during the stable middle and late stages,respectively.Additionally,six new components were detected in the cell membrane,along with a significant increase in both the types and concentration of fatty acids,thereby enhancing the stability of the phospholipid bilayer.This enhancement was the critical factor contributing to the strain's high tolerance to crude glycerol and its rapid growth.关键词
枯草芽孢杆菌/粗甘油耐受/分子机制解析/全基因组测序/转录组测序Key words
Bacillus subtilis/crude glycerol tolerance/analysis of molecular mechanisms/whole genome sequencing/RNA-sequencing分类
轻工纺织引用本文复制引用
贾晨阳,张帆,刘兰茜,王光路,杨雪鹏..一株高耐受粗甘油枯草芽孢杆菌的分子机制研究[J].轻工学报,2025,40(5):44-54,11.基金项目
河南省自然科学基金重点项目(242300421202) （242300421202）
河南省科技攻关重点研发与推广专项项目(232102311136) （232102311136）
