食品与发酵工业2025，Vol.51Issue(20)：1-8,8.DOI:10.13995/j.cnki.11-1802/ts.043026
调控磷酸戊糖途径非氧化支路提高毕赤酵母的甲醇利用率
Regulating non-oxidative pentose phosphate pathway genes to improve methanol utility of Komagataella phaffii
摘要
Abstract
The methylotrophic yeast Komagataella phaffii is a eukaryotic microorganism capable of utilizing methanol as sole carbon source,having been widely employed for protein and metabolite production.However,its endogenous methanol utilization efficiency re-quires further improvement.This study overexpressed encoding genes of key enzymes in the non-oxidative branch of the pentose phosphate pathway(PPP),including transketolase(tkt),ribulose-5-phosphate-3-epimerase(rpe),and fructose-1,6-bisphosphatase(fba),to en-hance methanol assimilation.Results showed that tkt-GS115 exhibited a 1.7％increase in OD600,with a maximum specific growth rate(μ)of 0.23 h-1.Compared to the control strain △FLDH,fba-△FLDH entered the growth phase 18 h earlier,achieving a similar μ of 0.23 h-1.Notably,rpe-△FDH and fba-△FDH rescued the growth defect of the parental strain △FDH in methanol minimal medium.Specif-ically,rpe-△FDH displayed significant growth potential,reaching a maximum OD600of 6.97,while fba-△FDH achieved a remarkably highμ of 0.36 h-1.Methanol utilization efficiency varied among the recombinant strains,including tkt-GS115,tkt-△FLDH,and fba-△FLDH showed improvements of 0.017,0.007,and 0.034,respectively.This study demonstrates that reinforcing three key genes in the PPP non-oxidative branch effectively enhances methanol utilization efficiency in K.phaffii recombinant strains.关键词
毕赤酵母/甲醇同化/磷酸戊糖途径/转酮醇酶/比生长速率Key words
Kamagataella phaffii/methanol assimilation/pentose phosphate pathway/transketolase/specific growth rate引用本文复制引用
臧秀,杨艳坤,白仲虎..调控磷酸戊糖途径非氧化支路提高毕赤酵母的甲醇利用率[J].食品与发酵工业,2025,51(20):1-8,8.基金项目
国家自然科学基金项目(32370054) （32370054）
