食品与发酵工业2025，Vol.51Issue(20)：9-16,8.DOI:10.13995/j.cnki.11-1802/ts.042038
基于L-墨角藻糖-1-磷酸醛缩酶在枯草芽孢杆菌中利用甘油合成D-阿洛酮糖
Synthesis of D-allulose from glycerol based on L-fuculose-1-phosphate aldolase in Bacillus subtilis
摘要
Abstract
D-allulose,an important rare sugar,serves as a low-calorie sweetener with the ability to regulate lipid metabolism and blood glucose balance.In Bacillus subtilis,a biosynthetic pathway from glycerol to D-allulose has been successfully constructed through heterologous expression of alditol oxidase(AldO),L-fuculose-1-phosphate aldolase(FucA),and fructose-1-phosphatase(YqaB).After optimizing the promoter of FucA,the ratio of mixed whole-cell was optimized.Then,to simplify the mixed whole-cell conversion system,a whole-cell conversion system using a single strain was constructed.Furthermore,the whole-cell conversion conditions,including tempera-ture,pH,whole-cell concentration,and glycerol concentration,were investigated.The optimal conditions for whole-cell conversion were 30 ℃,pH 6.5,whole-cell concentration 150 g/L,and glycerol concentration 50 g/L.Under optimized conditions,the production of D-al-lulose was achieved through glycerol feeding strategy,with the highest yield reaching 5.25 g/L,representing a 26.5％increase compared to the control group.This study presents a food-grade method for synthesizing D-allulose from glycerol.关键词
L-墨角藻糖-1-磷酸醛缩酶/D-阿洛酮糖/枯草芽孢杆菌/全细胞反应/启动子优化/甘油Key words
L-fuculose-1-phosphate aldolase/D-allulose/Bacillus subtilis/whole-cell conversion/promoter optimization/glycerol引用本文复制引用
费康清,陈洲,沈立群,中西秀树,李子杰..基于L-墨角藻糖-1-磷酸醛缩酶在枯草芽孢杆菌中利用甘油合成D-阿洛酮糖[J].食品与发酵工业,2025,51(20):9-16,8.基金项目
国家自然科学基金项目(32171475) （32171475）
江苏省自然科学基金项目(BK20210465) （BK20210465）
