食品与发酵工业2025，Vol.51Issue(20)：35-43,9.DOI:10.13995/j.cnki.11-1802/ts.041864
解脂亚洛酵母从头合成胭脂红酸
De novo biosynthesis of carminic acid in Yarrowia lipolytica
摘要
Abstract
Carminic acid,an aromatic polyketide compound,is a widely used natural red colorant.Traditionally,carminic acid is ex-tracted from cochineal insects,but the extraction process is labor-intensive and inefficient,leading to insufficient production to meet the growing market demand.In recent years,biosynthetic production of carminic acid has attracted significant attention.In this study,Yarrow-ia lipolytica was used as a host strain to achieve the de novo synthesis of carminic acid through the heterologous expression and screening of key pathway genes,resulting in a production titer of 0.7 mg/L.Since carminic acid biosynthesis requires two critical precursors,malo-nyl-CoA and acetyl-CoA,efforts were first made to enhance malonyl-CoA supply by screening ACC1 mutants and introducing a non-native malonyl-CoA biosynthetic pathway(NCM pathway),which increased the carminic acid titer to 1.2 mg/L.Subsequently,acetyl-CoA sup-ply was strengthened by regulating lipid metabolism,further boosting the titer to 1.5 mg/L.Additionally,by improving substrate utilization efficiency and relocating the carminic acid biosynthetic pathway to peroxisomes,the titer reached 1.9 mg/L.Finally,through fed-batch fermentation in a 5 L bioreactor,the production of carminic acid was scaled up to 9.6 mg/L(6.3 × 10-3mg/g).This study provides a ref-erence for the biosynthesis of carminic acid.关键词
胭脂红酸/丙二酰辅酶A/乙酰辅酶A/过氧化物酶体/解脂亚洛酵母Key words
carminic acid/malonyl-CoA/acetyl-CoA/peroxisomes/Yarrowia lipolytica引用本文复制引用
程俊译,李冬,曾伟主,周景文..解脂亚洛酵母从头合成胭脂红酸[J].食品与发酵工业,2025,51(20):35-43,9.基金项目
国家自然科学基金创新研究群体项目(32021005) （32021005）
2023年度江苏省卓越博士后计划项目(2023ZB282) （2023ZB282）
