沈阳农业大学学报2025，Vol.56Issue(5)：14-25,12.DOI:10.3969/j.issn.1000-1700.2025.05.002
水稻LEA基因家族的全基因组鉴定及综合分析
Genome-wide Identification and Comprehensive Analysis of LEA Gene Family in Rice
摘要
Abstract
[Objective]A large number of studies have shown that LEA proteins can help plants cope with environmental stress through various mechanisms.However,the functions of many LEA family members in rice have not been fully verified.Therefore,this study aimed to identify new LEA members involved in rice responses to abiotic stress through bioinformatics analysis of the OsLEA gene family and by combining their gene expression changes under different stresses.[Method]The OsLEA gene family was identified and analysed using a genome-wide bioinformatics approach.[Results]The results reveal that the rice genome contains 83 OsLEA family members,which are unevenly distributed across 12 chromosomes and can be classified into eight subfamilies based on structural domain features.Physicochemical property analysis shows that LEA proteins are highly hydrophilic and stable.Subcellular localization predictions indicate that most LEA proteins are localized in cellular compartments such as the cytoplasm,cell membrane,and mitochondria.Promoter cis-acting element analysis highlights that the OsLEA gene promoters are enriched with elements associated with abiotic stress response,phytohormone regulation,and growth and development.Tissue expression pattern and hormone response analyses reveal significant expression specificity across different tissues and under various phytohormone treatments.Additionally,certain OsLEA genes exhibit notable responses to abiotic stresses such as low and high temperatures,salt,and heavy metal(cadmium)treatments.These findings provide valuable insights into the role of OsLEA genes in coping with environmental stress.[Conclusion]This study integrated bioinformatics analysis with expression profiling data under multiple hormone treatments and abiotic stress conditions,preliminarily revealing the molecular regulatory mechanisms through which the OsLEA gene family participates in stress responses.Building on these findings,we successfully screened core member genes—including Os04g0589800,Os05g0362600,Os06g0324400,and Os08g0163600—which exhibited significant upregulation under high-salinity,high-temperature,and low-temperature stresses.This confirms their critical biological functions in abiotic stress responses and establishes them as highly promising molecular targets for breeding stress-resistant rice cultivars.关键词
水稻/LEA基因家族/非生物胁迫/启动子顺式作用元件/组织表达模式Key words
rice/LEA gene family/abiotic stress/promoter cis-acting elements/tissue expression pattern分类
农业科技引用本文复制引用
赵贺,冀亚亮,梁潇,阮班普,于彦春,马殿荣..水稻LEA基因家族的全基因组鉴定及综合分析[J].沈阳农业大学学报,2025,56(5):14-25,12.基金项目
辽宁省科技重大专项项目(2024JH1/11700006) （2024JH1/11700006）
