沈阳农业大学学报2025，Vol.56Issue(5)：44-51,8.DOI:10.3969/j.issn.1000-1700.2025.05.005
α-乳糖对弓形虫毒力株诱导的免疫病理的调节作用
Immunopathological Modulatory Effect of α-Lactose on Immune Pathology Induced by Virulent Strains of Toxoplasma gondii
摘要
Abstract
[Objective]Toxoplasma gondii,a protozoan parasite that can parasitize in the nucleated cells of almost all warm-blooded animals.Its infection can upregulate the expression of key exhaustion molecule,T cell immunoglobulin and mucin domain-containing protein 3(TIM-3)of host's spleen and peripheral blood T cells,thereby inhibiting the immune response.Previous studies showed that α-lactose can specifically regulate the interaction between TIM-3 and its ligand Galectin-9(Gal-9),thereby weakening its inhibitory function and enhancing host's anti-infection ability.This study aims to evaluate the immunopathological modulatory effects of α-lactose,mediated by its competitive binding to the TIM-3 ligand Gal-9,in mice infected with different virulent strains of Toxoplasma gondii(RH and ME49).To provide a new strategy for the immunomodulatory treatment of Toxoplasma gondii infection.[Methods]Using a Toxoplasma gondii-infected mouse model,mouse survival rates,spleen indices,and histopathological changes in the lungs and spleen were analyzed after α-lactose was administered via subcutaneous injection.Additionally,flow cytometry was used to assess the proportions of peripheral blood lymphocyte subsets and the expression of T cell immunoreceptor with Ig and ITIM domains(TIGIT)—an immune checkpoint molecule—on the cell surface.[Results]Although α-lactose treatment prolonged the survival time of mice infected with Toxoplasma gondii,it did not improve the spleen index or the histopathological damage in the lungs and spleens.Furthermore,α-lactose treatment did not significantly increase TIGIT expression on different peripheral blood lymphocyte subsets(including CD8⁺ T cells and NK cells)in mice following Toxoplasma gondii infection.[Conclusion]α-lactose did not significantly influence the mice infection with Toxoplasma gondii,which may be related to the virulence of the parasite strain and the state of host immune exhaustion.关键词
α-乳糖/弓形虫/免疫病理/淋巴细胞/TIGITKey words
α-lactose/Toxoplasma gondii/immune pathology/lymphocyte/TIGIT分类
农业科技引用本文复制引用
张义伟,李洪宇,景钰淇,孙露,姜宁..α-乳糖对弓形虫毒力株诱导的免疫病理的调节作用[J].沈阳农业大学学报,2025,56(5):44-51,8.基金项目
国家重点研发计划项目(2023YFD1802403) （2023YFD1802403）
沈阳农业大学公开招聘博士毕业生科研启动费项目(880421011) （880421011）
