中国食品添加剂2025，Vol.36Issue(11)：1-7,7.DOI:10.19804/j.issn1006-2513.2025.11.001
当归多糖的提取纯化及其基于斑马鱼模型的免疫调节功能研究
Extraction and purification of polysaccharides from Angelica and their immunoregulation effects based on a zebrafish model
摘要
Abstract
In order to optimize the extraction and purification process of Angelica sinensis polysaccharide and evaluate their immune regulation functions.The extraction yield of Angelica polysaccharide was used as the primary index.The extraction conditions were optimized via single-factor and orthogonal experiments,followed by purification of the extracts.The immunoregulatory effects of different purified polysaccharide fractions were then investigated.The results showed that the optimal extraction process was as follows:crushing Angelica sinensis,adding 3%cellulase and 150 times pure water,adjusting the pH to 5 with citric acid,and extracted at 50℃for 2.5 h.Under the above conditions,the polysaccharide extraction yield was(36.71±0.38)%.The crude polysaccharide was separated and purified to obtain two main polysaccharide fractions:APS-1 and APS-2.Immunomodulatory assays demonstrated that both APS-1 and APS-2 significantly enhanced the phagocytic activity of zebrafish macrophages,with the effective concentration was about 50 μg/mL.Thus,the method employed in this study was demonstrated to efficiently extract and purify Angelica polysaccharides,The obtained Angelica polysaccharide fractions demonstrated notable immunomodulatory activity,providing data support for the extraction,purification and functional research of Angelica polysaccharide in the future.关键词
当归/多糖/提取纯化/免疫调节/细胞吞噬Key words
Angelica sinensis/polysaccharides/extraction and purification/immunoregulation/phagocytosis分类
轻工业引用本文复制引用
石万银,赖富丽,崔灵敏,唐万,陈阳,王喆,杨强,徐剑..当归多糖的提取纯化及其基于斑马鱼模型的免疫调节功能研究[J].中国食品添加剂,2025,36(11):1-7,7.基金项目
湖北省自然科学基金项目(2022CFD077) （2022CFD077）
