中国食品添加剂2025，Vol.36Issue(11)：16-25,10.DOI:10.19804/j.issn1006-2513.2025.11.003
超声辅助纤维素酶法提取天麻多酚的工艺优化及体外活性研究
Optimization of ultrasound-assisted cellulase extraction of polyphenols from Gastrodia elata and evaluation of their activity in vitro
摘要
Abstract
This study aimed to optimize the extraction process of Gastrodia elata polyphenols and evaluate their bioactivities in vitro.Fresh G.elata was used as the raw material.Single-factor experiments were first conducted to examine the key variables influencing polyphenol extraction efficiency.Subsequently,response surface methodology was applied to determine the optimal conditions for ultrasonic-assisted cellulase extraction.The optimized extraction parameters were:liquid-to-material ratio of 31:1,ethanol concentration of 73%,cellulase content of 1.3%,and ultrasonic power of 290 W,resulting in a polyphenol extraction rate of 0.62%.In vitro activity assays revealed that the half-maximal inhibitory concentrations(IC50)for ABTS+,DPPH,and hydroxyl radicals were 43.44,65.75,and 31.15 μg/mL,respectively.Regarding glucose-lowering activity,the IC50 values for α-amylase and α-glucosidase inhibition were 41.29 and 22.40 μg/mL,respectively.These findings provided a useful reference for the efficient extraction and in vitro activity evaluation of G.elata polyphenols using ultrasound-assisted cellulase methods.关键词
天麻多酚/体外抗氧化/降血糖/超声提取Key words
Gastrodia elata polyphenols/in vitro antioxidant/hypoglycemic/ultrasonic-assisted extraction分类
轻工业引用本文复制引用
盛婧,许美铃,董仕豪,郭磊..超声辅助纤维素酶法提取天麻多酚的工艺优化及体外活性研究[J].中国食品添加剂,2025,36(11):16-25,10.基金项目
云南省大学生创业训练项目(S202410677100) （S202410677100）
云南省天麻与真菌共生生物学重点实验室开放课题(TMKF2024B05) （TMKF2024B05）
