中国食品添加剂2025，Vol.36Issue(11)：26-31,6.DOI:10.19804/j.issn1006-2513.2025.11.004
砂仁提取物活性成分及其对黄嘌呤氧化酶抑制活性研究
Study on active compounds in Amomum villosum extract and their inhibitory activity on xanthine oxidase
摘要
Abstract
This study utilized Amomum villosum as the raw material to prepare six different specifications of extracts through various extraction methods.The differences in the content of main active components among the six extracts were analyzed.Based on a high-throughput screening model,the inhibitory effects of the six extracts on xanthine oxidase were evaluated through in vitro enzyme activity assays.The results indicated that there were significant differences(P<0.05)in the active component content among the six Amomum villosum extracts.The extracts primarily composed of total polysaccharides,total polyphenols,and total flavonoids all exhibited inhibitory activity against xanthine oxidase,with the total flavonoid extract demonstrating the highest inhibitory activity.Within a certain concentration range,the content of total flavonoids in the Amomum villosum extract showed a positive correlation with its xanthine oxidase inhibitory activity.When the concentration of the extract(total flavonoids≥50%)reached 0.8 mg/mL,its inhibitory activity against xanthine oxidase was comparable to that of the positive control group(allopurinol),achieving an inhibition rate of(98.98±0.13)%.The results provide a foundation for the development and utilization of Amomum villosum extract in functional food for uric acid reducing.关键词
砂仁/乙酸龙脑酯/总多糖/总黄酮/黄嘌呤氧化酶/降尿酸Key words
Amomum villosum/bornyl acetate/total polysaccharides/total flavonoids/xanthine oxidase/uric acid reducing分类
轻工业引用本文复制引用
彭婷,陈志元,赖富丽,陈阳,王玉,陈存..砂仁提取物活性成分及其对黄嘌呤氧化酶抑制活性研究[J].中国食品添加剂,2025,36(11):26-31,6.基金项目
湖北省重点研发计划项目(2022ACA003-03-004) （2022ACA003-03-004）
黄石市揭榜制科技项目(2023A005) （2023A005）
