中国食品添加剂2025，Vol.36Issue(11)：48-58,11.DOI:10.19804/j.issn1006-2513.2025.11.007
产圆柚酮菌种筛选及发酵条件优化
Screening of nootkatone-producing strains and optimization of fermentation conditions
摘要
Abstract
To use biotransformation method to produce non-toxic nootkatone,strains were isolated from the navel orange peels rich in valencene and nootkatone.Through primary and secondary screening,strains capable of converting valencene into nootkatone were selected.The screened strains were identified by 16S rDNA sequencing and subjected to genetic stability tests and fermentation condition optimization.The results indicated that the most efficient strain was identified as Starmerella sorbosivorans.The optimal fermentation conditions were as follows:4.00 g/L Tween 80,30.00 μL valencene,pH 4.00,0.50 g/L ferrous sulfate,30.00 g/L malic acid and fermentation duration 48 h.Under the above conditions,the highest nootkatone yield reached 60.58 mg/L.Furthermore,the presence of magnesium ions was found to inhibit nootkatone synthesis by Starmerella sorbosivorans from valencene,while ferrous ions promoted it.These findings provide a foundation for the industrial production of nootkatone.关键词
圆柚酮/瓦伦西亚橘烯/山梨木糖假丝酵母/生物转化Key words
nootkatone/valencene/Starmerella sorbosivorans/biotransformation分类
轻工业引用本文复制引用
潘奕丞,刘晓旋,钱敏,王启轩,白卫东,赵文红..产圆柚酮菌种筛选及发酵条件优化[J].中国食品添加剂,2025,36(11):48-58,11.基金项目
功能性内酯与手性香料绿色制备关键技术研发与示范(2022B0202050003) （2022B0202050003）
仲恺农业工程学院研究生创新基金(KJCX2024017) （KJCX2024017）
