中外葡萄与葡萄酒Issue(6)：42-50,9.DOI:10.13414/j.cnki.zwpp.2025.06.006
UPLC测定葡萄与葡萄酒中花色苷的方法研究
UPLC Study for Determination of Anthocyanins in Grapes and Wine
摘要
Abstract
The objective of the present study was to optimise an ultra performance liquid chromatography(UPLC)method for the determination of seven anthocyanins in grapes and wines.Following a rigorous comparison of the separation effects exhibited by the Shim-pack GIST C18 column(2.1 mm×100 mm,2 μm)and the Kinetex PFP column(3 mm×100 mm,2.7 μm),it was determined that the latter was to be selected as the stationary phase.The grape samples were extracted using formic acid:methanol(2∶98,V/V),and the wine samples were injected directly.The mobile phase A was composed of formic acid in water,with a volume ratio of 3∶97,while mobile phase B was a mixture of formic acid and acetonitrile,with a volume ratio of 2∶98.The separation process was conducted in gradient elution mode,with a detection wavelength of 518 nm and an injection volume of 1 μL.It was demonstrated that under optimised chromatographic conditions,the seven anthocyanosides were successfully separated within 11 minutes.Furthermore,an excellent linear relationship was observed between the concentration and the peak area(R2＞0.9981).The range of recoveries was from 87.3%to 92.91%,with a precision(RSD)value below 1%.The method has been demonstrated to facilitate rapid and accurate qualitative and quantitative analysis of anthocyanosides in grapes and wines.It is characterised by high reliability and significant application value.关键词
超高效液相色谱/葡萄/葡萄酒/花色苷/方法优化Key words
ultra high liquid chromatography/grape/wine/anthocyanins/method optimization分类
园艺学与植物营养学引用本文复制引用
梁艳英,谭鸿冰,张梦博,王利,张予林,蒋娟..UPLC测定葡萄与葡萄酒中花色苷的方法研究[J].中外葡萄与葡萄酒,2025,(6):42-50,9.基金项目
西北农林科技大学"新工科"本科教学实验室开放与实验项目开发研究(JY2303115)西北农林科技大学实验技术研究与实验管理创新项目(Sy20230216) （JY2303115）
