食品工业科技2025，Vol.46Issue(23)：11-22,12.DOI:10.13386/j.issn1002-0306.2025010220
玉米胚芽粕降血糖功能肽的制备及其体外降血糖活性
Preparation of Hypoglycemic Functional Peptides from Corn Germ Meal and Its Hypoglycemic Activity in Vitro
摘要
Abstract
In order to investigate the optimal preparation process and in vitro hypoglycemic activity of functional peptides from corn germ meal(CGMPs),this study used corn germ meal as the raw material,and the inhibition rates of dipeptidyl peptidase-Ⅳ(DPP-Ⅳ)and α-glucosidase as indicators.CGMPs were prepared by enzymatic hydrolysis with alcalase.The preparation of CGMPs was optimized using single-factor experiments and response surface methodology.On this basis,the enzymatic reaction kinetics of CGMPs and their inhibitory effects on DPP-Ⅳ activity in Caco-2 cells were analyzed.The optimal enzymatic hydrolysis conditions were as follows:Substrate concentration of corn germ meal 4％,enzyme dosage of alcalase 1003 U/g,enzymatic hydrolysis pH7.5,enzymatic hydrolysis temperature 59.5 ℃,and reaction time 3 h.Under these conditions,the inhibition rates of CGMPs on α-glucosidase and DPP-Ⅳ were 75.74％±2.02％and 61.64％±2.07％(1 mg/mL),respectively.The enzymatic reaction kinetics results showed that the inhibition of CGMPs on DPP-Ⅳ was competitive and reversible.In addition,CGMPs also exhibited good DPP-Ⅳ inhibitory ability at the in vitro cellular level,indicating that they could be used as a functional food ingredient for the development of hypoglycemic functional foods.This study provides a theoretical basis for the high-value utilization of corn germ meal protein resources.关键词
玉米胚芽粕/碱性蛋白酶/α-葡萄糖苷酶/二肽基肽酶-Ⅳ(DPP-Ⅳ)/降血糖活性Key words
corn germ meal/alcalase/α-glucosidase/dipeptidyl peptidase-Ⅳ/hypoglycemic activity分类
轻工纺织引用本文复制引用
高翔,刘晓兰,李冠龙,郑喜群..玉米胚芽粕降血糖功能肽的制备及其体外降血糖活性[J].食品工业科技,2025,46(23):11-22,12.基金项目
黑龙江省自然科学基金(PL2024C039) （PL2024C039）
中央引导地方科技发展专项(ZY23QY06) （ZY23QY06）
黑龙江省"双一流"建设学科协同创新成果建设项目(培育项目)(LJGXCG2023-104) （培育项目）
黑龙江省"双一流"新一轮建设学科协同创新成果建设项目(孵化项目)(LJGXCG2023-055). （孵化项目）
