食品工业科技2025，Vol.46Issue(23)：70-80,11.DOI:10.13386/j.issn1002-0306.2024110123
酸/碱提取菠萝蜜果丝多糖及其结构、生物活性的影响
Effect of Acid/Alkali Extraction of Polysaccharides from Jackfruit Filum and Its Structure and Biological Activity
摘要
Abstract
This experiment focused on the preparation of polysaccharides from jackfruit filum using both acid and alkaline extraction methods.After determining the polysaccharide yield,the structures of the polysaccharides were analyzed and compared using various techniques,including high-performance liquid chromatography,Fourier-transform infrared spectroscopy,ion chromatography,nuclear magnetic resonance,and differential scanning calorimetry.The biological properties of the polysaccharides were evaluated through in vitro assessments of their antioxidant and hypoglycemic activities.The results indicated that the yield of acid-extracted polysaccharide(AcJFP)was lower than that of alkali-extracted polysaccharide(AlJFP).Both AcJFP and AlJFP fractions were composed of monosaccharides such as glucose and galacturonic acid.The weight-average molecular weights were 1.01 kDa for AcJFP and 12.08 kDa for AlJFP.Both fractions contained α and β glycosidic linkages,together with long side chains and numerous branches,and lacked a triple-helical structure.X-ray diffraction patterns revealed differences in the crystalline aggregation structures of the two polysaccharides,while thermogravimetric analysis demonstrated good thermal stability.In vitro biological activity assessments showed that AcJFP exhibited stronger DPPH,hydroxyl,ABTS+radical-scavenging abilities,total antioxidant capacity,and inhibition of α-amylase activity,while AlJFP showed superior ferrous ion-chelating ability.Therefore,the method of extraction impacts both the structure and biological activities of polysaccharides from jackfruit filum.关键词
菠萝蜜果丝/多糖/pH/结构表征/抗氧化/降血糖Key words
jackfruit filum/polysaccharide/pH/structural characterization/antioxidant/hypoglycemic分类
轻工纺织引用本文复制引用
李智聪,李俊,熊晓萱,丁金休,唐碧怡,黎根瑚,陈其勇,林日辉..酸/碱提取菠萝蜜果丝多糖及其结构、生物活性的影响[J].食品工业科技,2025,46(23):70-80,11.基金项目
国家自然科学基金项目(21766004) （21766004）
广西自然科学基金(2019GXNSFAA185008) （2019GXNSFAA185008）
广西民族大学研究生教育创新计划(gxmzu-chxs2024232). （gxmzu-chxs2024232）
