食品工业科技2025，Vol.46Issue(23)：221-228,8.DOI:10.13386/j.issn1002-0306.2025070093
高效降解嘌呤核苷乳酸菌筛选及益生特性研究
Screening of Lactic Acid Bacteria with High Efficiency in Degrading Purine Nucleotides and Study on Probiotic Characteristics
摘要
Abstract
To screen Lactobacilli strains that exhibit efficient purine nucleoside degradation capability and demonstrate probiotic potential,this study screened target strains from 88 lactic acid bacteria(LAB)strains using nucleoside degradation ability as an indicator.Consequently,eight strains with nucleoside degradation rates exceeding 80％were isolated.Further evaluations of their tolerance to simulated intestinal fluid and adhesion to Caco-2 cells led to the selection of strain CM3 3.This strain demonstrated exceptional nucleoside degradation performance,with inosine and guanosine degradation rates of 99.83％and 99.92％.Its tolerance to simulated intestinal fluid reached 96.82％,and its adhesion rate to Caco-2 cells was 61.36％.Identification via 16S-rDNA sequencing confirmed that CM33 belonged to the species Limosilactobacillus fermentum.However,strain CM33 exhibited limited tolerance to environments with pH levels of 2.5 and 3.0.This research provides a theoretical foundation for the development of LAB preparations with high purine nucleoside degradation capability.关键词
嘌呤核苷/嘌呤代谢紊乱/尿酸/乳酸菌/黏附定植Key words
purine nucleoside/purine metabolism disorder/uric acid/lactic acid bacteria/adhesion and colonization分类
轻工业引用本文复制引用
胡旺,门光明,姜梅..高效降解嘌呤核苷乳酸菌筛选及益生特性研究[J].食品工业科技,2025,46(23):221-228,8.基金项目
2025年国家级大学生创新创业训练计划(X2025103070364). （X2025103070364）
