食品工业科技2025，Vol.46Issue(23)：302-309,8.DOI:10.13386/j.issn1002-0306.2024070256
基于Co3O4-NC的过氧化氢酶活性比色检测茶叶中的没食子酸
Colorimetric Detection of Gallic Acid in Tea Based on the Catalase-like Activity of Co3O4-NC Nanozyme
摘要
Abstract
Objective:The present study was aimed to develop a rapid colorimetric method for the detection of gallic acid in tea based on the catalase-like activity of Co3O4-NC nanozyme.Methods:The Co3O4-NC was prepared by calcination of the composite material of zeolitic imidazolate frameworks-67(ZIF-67)and polydopamine.The letramethylbenzidine(TMB)could be oxidized to blue oxTMB in the presence of H2O2 by Co3O4-NC nanozyme,while the addition of gallic acid would restrain the catalase-like activity and the color of the solution will be lighter.Based on the mechanism,a colorimetric sensing platform for the detection of gallic acid was established.Results:The linear curve between ΔA and GA concentration was observed within the range of 0.25～20.00 μmol/L,the linear equation was ΔA=0.0310C+0.0384(R2=0.995),the limit of detection(LOD,3v/S)was 0.077 μmol/L and the limit of quantitation(LOQ,10σ/S)was 0.26 μmol/L.Conclusion:The method could be applied to detect gallic acid equivalent(GAE)in tea,and the GAE in green tea,black tea and oolong tea were tested as 2.33％,1.82％,and 0.46％,respectively.The recovery rates ranged from 90.97％to 105.22％with RSD＜5％,indicating that the colorimetric method had a good application prospect.关键词
纳米酶/Co3O4-NC/过氧化氢酶/比色检测/没食子酸Key words
nanozyme/Co3O4-NC/catalase-like activity/colorimetric detection/gallic acid分类
轻工业引用本文复制引用
胡晓霞,刘晓丹,许萍,邢彦军,刘小杰..基于Co3O4-NC的过氧化氢酶活性比色检测茶叶中的没食子酸[J].食品工业科技,2025,46(23):302-309,8.基金项目
山东省博士后创新基金(202103067) （202103067）
上海城建职业学院校级一般项目(cjky202310). （cjky202310）
