食品与发酵工业2025，Vol.51Issue(22)：10-18,9.DOI:10.13995/j.cnki.11-1802/ts.042204
基于比较基因组学解析罗伊氏粘液乳杆菌FQ56对小鼠高尿酸血症的缓解机制
Exploring mechanism of hyperuricemia relief in mice by Limosilactobacillus reuteri FQ56 via comparative genomics
摘要
Abstract
This study aimed to explore the mechanism by which Limosilactobacillus reuteri FQ56 alleviates hyperuricemia in mice through comparative genomics analysis.A total of 24 male Kunming mice,aged 5 weeks,were selected for the study and were given daily intervention with L.reuteri at a dose of 109 CFU.For the final two weeks,hyperuricemia was induced by the uricase inhibitor potassium ox-onate and hypoxanthine.At the end of the experiment,serum uric acid levels,xanthine oxidase(XOD)activity,kidney damage,and changes in gut microbiota and short-chain fatty acids(SCFAs)levels were assessed.Results showed that L.reuteri FQ56 significantly re-duced serum uric acid levels in the mice,inhibited the activity of XOD in both the liver and serum,and alleviated kidney damage induced by hyperuricemia.Additionally,L.reuteri FQ56 increased the abundance of SCFA-producing bacteria,such as Roseburia and Eubacterium_xylanophilum,and raised SCFA concentrations in the feces of the mice.Comparative genomics analysis revealed that the FQ56 strain pos-sesses a greater number of glycoside hydrolase genes,particularly from the GH1 family.These genes may help regulate gut microbiota com-position and promote the hydrolysis of polysaccharides in the gut,thereby stimulating SCFA production in the colon and inhibiting XOD activity in both serum and liver,ultimately alleviating hyperuricemia.This study provides new insights into the mechanisms by which pro-biotics alleviate hyperuricemia,particularly the role of glycoside hydrolase genes in this process,offering a theoretical basis for future re-search and clinical applications.关键词
罗伊氏粘液乳杆菌/高尿酸血症/短链脂肪酸/肠道菌群/比较基因组学/糖苷水解酶Key words
Limosilactobacillus reuteri/hyperuricemia/short-chain fatty acids/gut microbiota/comparative genomics/glycoside hydro-lase引用本文复制引用
孙磊,赵建新,陈卫,王刚..基于比较基因组学解析罗伊氏粘液乳杆菌FQ56对小鼠高尿酸血症的缓解机制[J].食品与发酵工业,2025,51(22):10-18,9.基金项目
国家自然科学基金项目(32272332) （32272332）
