食品与发酵工业2025，Vol.51Issue(22)：89-100,12.DOI:10.13995/j.cnki.11-1802/ts.042076
食源唾液酸糖肽糖基化修饰对病原微生物和老人肠道菌群的影响
Impact of dietary sialoglycopeptides glycosylation on pathogenic microorganisms and gut microbiota in elderly individuals
摘要
Abstract
Protein glycosylation modulates the structural properties and biological functions of glycoproteins.Edible bird's nest(EBN)and casein glycomacropeptide(CGMP)are both highly sialylated glycoprotein and glycopeptide.EBN is characterized by the pres-ence of both N-glycan and O-glycan modifications,whereas CGMP contains exclusively O-glycans.This study compared the biological functions of EBN glycopeptides and CGMP,including their inhibitory effects on pathogens such as Candida albicans,Helicobacter pylori,and avian influenza virus(AIV),as well as their modulatory effects on the structure of the intestinal flora in elderly individuals.The con-trols used in this study include desialylated EBN glycopeptides,N-acetylneuraminic acid,and 3′-sialyllactose.DNA sequencer-based fluo-rescent glycan electrophoretic analysis revealed that EBN contained multiantennary N-glycans with sialylation and bisecting N-acetylglu-cosamine glycan chains.Results indicated that EBN glycopeptides could significantly inhibit C.albicans and H.pylori compared to CGMP.This inhibitory activity was substantially diminished when the terminal sialic acid residues of the EBN glycopeptides were removed(P＜0.05).It was also found that EBN glycopeptides could effectively inhibit the adhesion of AIV to MDCK cells.In vitro fecal fermentation u-sing fecal samples from elderly individuals demonstrated that EBN glycopeptides exhibited remarkable probiotic effects on the gut microbio-ta.In conclusion,the sialylation structure of glycoproteins played a crucial role in determining their biological activities.EBN glycopep-tides,which were rich in both sialylated N-glycans and O-glycans,demonstrated a robust ability to inhibit pathogenic microorganisms and exhibited superior probiotic properties compared to CGMP that contained only O-glycosylation.关键词
N-糖基化/膳食唾液酸糖肽/病原菌/禽流感病毒/肠道菌群Key words
N-glycosylation/dietary sialoglycopeptide/pathogen/avian influenza virus/gut microbiota引用本文复制引用
朱娇,吴剑荣,牛文璇,范群艳,柳训才..食源唾液酸糖肽糖基化修饰对病原微生物和老人肠道菌群的影响[J].食品与发酵工业,2025,51(22):89-100,12.基金项目
国家重点研发计划项目(2023YFA0914303) （2023YFA0914303）
