食品与发酵工业2025，Vol.51Issue(22)：145-153,9.DOI:10.13995/j.cnki.11-1802/ts.042092
格氏乳杆菌G098的全基因组解析及培养基优化
Genome-wide analysis and medium optimization of Lactobacillus gasseri G098
摘要
Abstract
Lactobacillus gasseri G098,isolated from the intestine of a healthy infant in the Tibet,China,demonstrates excellent pro-biotic properties,including strong tolerance to artificial gastrointestinal fluids and bile salts.However,its low cell density during cultiva-tion limits its potential for industrial production.To address this limitation,this study optimized the culture medium for L.gasseri G098 by genomic information for rapid screening of growth factors.Whole-genome sequencing analysis revealed that the genome of L.gasseri G098 spans 1 991 131 bp,with a GC content of 35.30％,and encodes 1 982 genes.Comprehensive metabolic pathway analysis of the genomic data identified specific amino acids and vitamins critical for growth,which were incorporated into subsequent medium optimization experi-ments.The final optimized medium formulation consisted of the following components(per liter):38.00 g lactose,62.66 g fish peptone,31.33 g tryptone,5.75 g dipotassium phosphate,14.27 g anhydrous sodium acetate,5.75 g sodium citrate,0.01 g FeSO4·7H2O,and 1.00 g Tween 80,dissolved in 1 000 mL of distilled water.Cultivation of L.gasseri G098 in this optimized medium resulted in a viable cell count of 5.48 × 109 CFU/mL,representing a 5.1-fold increase compared to the unoptimized medium.关键词
格氏乳杆菌/基因组学/培养基优化Key words
Lactobacillus gasseri/genomics/medium optimization引用本文复制引用
康嘉禾,周亮,谢佳琪,赵洁..格氏乳杆菌G098的全基因组解析及培养基优化[J].食品与发酵工业,2025,51(22):145-153,9.基金项目
内蒙古自治区直属高校基本科研业务费项目(BR230406) （BR230406）
国家自然科学基金地区基金项目(32260573) （32260573）
