食品与发酵工业2025，Vol.51Issue(22)：247-253,7.DOI:10.13995/j.cnki.11-1802/ts.041686
产蛋白酶苏云金芽孢杆菌的筛选及其在甲壳素制备中的应用
Screening of protease-producing Bacillus thuringiensis and its application in chitin preparation
摘要
Abstract
To address the environmental impact of conventional chemical processes,this study developed a sustainable method for chitin extraction.A high protease-producing strain of Bacillus thuringiensis LX-W4 was isolated from naturally fermented crayfish shells and co-fermented with Enterococcus faecalis LX18-9.The fermentation conditions(including initial pH,temperature,crayfish shell addition and duration)were optimised,and the optimal parameters were determined to be pH 6.0,fermentation temperature 40℃,crayfish shell addition 6％and fermentation for 4 days.Under these conditions,the protease activity of LX-W4 was 86.02 U/mL and the deproteinisati-on rate reached 95.21％.Structural characterisation using scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FT-IR),and X-ray diffraction(XRD)confirmed that LX-W4 had an α-crystal structure with a deacetylation degree of 15％and a crys-tallinity index of 84.8％.The surface morphology was smooth,flat,and porous,with higher purity and structural integrity compared with chemically extracted chitin.Compared with the traditional chemical method,the microbial fermentation method better preserved the molec-ular structure of chitin and improved the purity of the product.This study establishes a novel fermentation process for chitin extraction from crayfish shells,which provides a viable method for the high-value utilisation and recycling of crayfish by-products.The results of the study highlight the potential of microbial fermentation as an environmentally friendly alternative method for chitin production.关键词
小龙虾壳/蛋白酶/菌株筛选/发酵/甲壳素Key words
crayfish shell/protease/strain screening/fermentation/chitin引用本文复制引用
阚嘉,江宁,王鑫,王成,梁丽雅,盘赛昆,马艳弘..产蛋白酶苏云金芽孢杆菌的筛选及其在甲壳素制备中的应用[J].食品与发酵工业,2025,51(22):247-253,7.基金项目
江苏省农业科技自主创新项目[SCX(23)3185] （23）
中国博士后科学基金项目(2023M741457) （2023M741457）
