食品与发酵工业2025，Vol.51Issue(22)：411-419,9.DOI:10.13995/j.cnki.11-1802/ts.043507
抗菌肽微生物异源表达系统和表达策略优化研究进展
Research advances on heterologous expression systems and expression strategies optimization for antimicrobial peptides
摘要
Abstract
Antimicrobial peptides(AMPs)are a class of polypeptides characterized by excellent antibacterial activity,remarkable stability,low propensity for inducing drug resistance and high biological safety,these attributes render them promising alternatives to con-ventional antibiotics.Presently,there is three production methods that employed for AMPs production,including bio-extraction,chemical synthesis,and gene engineering expression.Microbial heterologous expression is one of gene engineering expression methods,has garnered significant attention.As a novel preparation method,microbial heterologous expression offers distinct advantages in terms of economy,con-venience,and efficiency,thereby emerging as a crucial strategy for the large-scale production of AMPs.The selection of heterologous ex-pression systems and expression optimization strategies are pivotal factors influencing the successful large-scale production of AMPs.This paper systematically reviews the development of microbial heterologous expression systems for AMPs and further elaborates on the optimiza-tion strategies of microbial heterologous expression,providing a comprehensive overview on the current state of this field.Additionally,this review offers insights and prospects for future advancements in microbial heterologous expression.The objective is to provide both theoreti-cal and practical guidelines for the efficient expression and large-scale production of AMPs.关键词
抗菌肽/微生物异源表达/真核表达系统/原核表达系统/表达策略Key words
antimicrobial peptides/microbial heterologous expression/eukaryotic expression system/prokaryotic expression system/ex-pression strategies引用本文复制引用
李玉珍,肖怀秋,王斌,廖琼,刘畅宇,唐旭阳..抗菌肽微生物异源表达系统和表达策略优化研究进展[J].食品与发酵工业,2025,51(22):411-419,9.基金项目
湖南省自然科学基金科教联合基金项目(2022JJ60046) （2022JJ60046）
