食品与机械2025，Vol.41Issue(10)：1-10,10.DOI:10.13652/j.spjx.1003.5788.2025.80007
乳酸片球菌N8的益生特性及高活性保护剂的筛选
Probiotic properties of Pediococcus acidilactici N8 and screening of high-viability protectants
摘要
Abstract
[Objective]To investigate the biological characteristics and in vitro probiotic functions of the food-derived Pediococcus acidilactici N8,and to screen effective protectants for the preparation of high-viability(>1012 CFU/g)spray-dried probiotic preparations.[Methods]A lactic acid bacterium(strain N8)isolated from cheese was identified through morphological analysis and 16S rDNA gene sequencing.Its probiotic potential was determined through tests on growth characteristics,safety,tolerance,antibacterial activity,adhesion ability,and antioxidant capacity.Spray drying was employed to prepare probiotic formulations,and high-viability spray-drying protectants were screened.[Results]Based on morphological characteristics and 16S rDNA gene sequence analysis,strain N8 was identified as P.acidilactici.It exhibited a rapid S-shaped growth curve and probiotic traits including non-hemolytic activity and sensitivity to multiple antibiotics.Its supernatant effectively inhibited the growth of various pathogenic bacteria.The strain showed strong stability under heat,varying pH levels,bile salts,and simulated gastrointestinal fluid conditions,and possessed strong adhesion ability.Both the cell-free supernatant and intact cell suspension of P.acidilactici N8 demonstrated strong scavenging abilities against DPPH,ABTS,and hydroxyl radicals.When buffalo milk,Cordyceps militaris polysaccharides,and sodium glutamate were used as protectants,the viable count of the probiotic preparation reached 1012 CFU/g.[Conclusion]P.acidilactici N8 is a food-derived lactic acid bacterium with good probiotic potential.Using buffalo milk,C.militaris polysaccharides,and sodium glutamate as spray-drying protectants can yield high-viability probiotic preparations.关键词
乳酸片球菌/益生特性/抗氧化特性/高活性/喷雾干燥Key words
Pediococcus acidilactici/probiotic properties/antioxidant properties/high-viability/spray drying引用本文复制引用
赵瑛,邹苑,叶志伟,郭丽琼,林俊芳..乳酸片球菌N8的益生特性及高活性保护剂的筛选[J].食品与机械,2025,41(10):1-10,10.基金项目
广东省重点领域研发计划"营养健康食品创制"专项项目(编号:2022B0202050002) （编号:2022B0202050002）
国家自然科学基金项目(编号:32272785,32072646) （编号:32272785,32072646）
