食品与机械2025，Vol.41Issue(10)：27-33,7.DOI:10.13652/j.spjx.1003.5788.2024.81316
基于Ag/g-C3N4纳米酶检测牛奶中过氧化氢
Detection for hydrogen peroxide in milk by the nanozyme based on Ag/g-C3N4
摘要
Abstract
[Objective]To develop a colorimetric analysis method for the rapid detection of H2O2 in milk.[Methods]A novel composite nanozyme material,Ag/g-C3N4,is synthesized by loading nano-Ag on the surface of g-C3N4 nanosheets via a reduction method.The structure and morphology of the material are characterized by X-ray diffraction and transmission electron microscopy.The catalytic conditions for the TMB-H2O2 colorimetric reaction are optimized,and catalytic mechanisms and steady-state kinetics are analyzed.[Results]The Ag/g-C3N4 material exhibits good peroxidase-like activity,and the enzyme-catalyzed reaction follows the Michaelis-Menten kinetic model.The optimal detection range for H2O2 in the colorimetric system is 10～50 μmol/L,with a detection limit of 0.05 μmol/L.When applied to detect H2O2 in real milk samples,the recovery rate is between 99.47%and 102.83%.[Conclusion]The developed colorimetric analysis method is simple to prepare,cost-effective,highly sensitive,and demonstrates good operability and practicality.关键词
Ag/g-C3N4/纳米酶/比色分析/H2O2Key words
Ag/g-C3N4/nanozyme/colorimetric analysis/H2O2引用本文复制引用
朱雁风,王松楠..基于Ag/g-C3N4纳米酶检测牛奶中过氧化氢[J].食品与机械,2025,41(10):27-33,7.基金项目
河南省科技攻关计划项目(编号:222102230033) （编号:222102230033）
河南科技大学实验技术开发项目(编号:SY2425040) （编号:SY2425040）
