中国食物与营养2025，Vol.31Issue(11)：1-8,8.
体外模拟消化结合结肠发酵技术探究BB12+GOS&FOS婴幼儿配方奶粉对肠道菌群的影响
Effects of BB12+GOS&FOS Infant Formulas on Gut Microbiota Via in Vitro Simulated Digestion Combined with Colonic Fermentation
肖杰 1王黎明 1逄金柱 2安泰 1侯粲 1贾健斌 1李居南3
作者信息
- 1. 中粮营养健康研究院有限公司,北京 102209||营养健康与食品安全北京市重点实验室,北京 102209
- 2. 内蒙古蒙牛乳业(集团)股份有限公司,北京 101107
- 3. 内蒙古蒙牛乳业(集团)股份有限公司,北京 101107||贝拉米食品贸易(上海)有限公司,上海 201306
- 折叠
摘要
Abstract
[Objective]This study was conducted to investigate the difference of BB12+GOS&FOS infant formulas and prebiotic added infant formulas on gut microbiota via in vitro simulated digestion combined with colonic fermentation.[Method]The survival rate of BB12 was determined after simulating gastrointestinal diges-tion.Both BB12+GOS&FOS infant formula and GOS infant formula were simulated gastrointestinal digestion and fecal microbial fermentation.Gut microbiota relative abundance was analyzed using intestinal microbial sequencing technology.Meanwhile,short-chain fatty acid(SCFA)contents were measured.The underlying causes of SCFAs production were analyzed,and the microbial metabolism pathway were predicted.[Result]The survival rate of BB12 after gastrointestinal digestion was(72.88±8.88)%approximately.Analysis of the gut microbiota revealed that intervention with BB12 combined with GOS and FOS significantly increased the propor-tion of Veillonella,rising from 7.6%to 42.1%.Additionally,the concentrations of acetic acid,propionic acid,and butyric acid increased to(196.80±68.19)μmol/mL,(50.05±7.95)μmol/mL,and(9.87±1.84)μmol/mL,respectively.[Conclusion]This study showed that BB12+GOS&FOS intervention can improve the abundance of Veillonella and the content of SCFAs,which could enhance colon barrier and intestinal immunity.Inflammatory response was weakened and the development of intestinal barrier was promoted via inhibiting Th17 cell dif-ferentiation and IL-17 signaling pathway.关键词
模拟消化/结肠发酵/益生菌/益生元/短链脂肪酸Key words
simulated digestion/colonic fermentation/probiotic/prebiotic/short chain fatty acid引用本文复制引用
肖杰,王黎明,逄金柱,安泰,侯粲,贾健斌,李居南..体外模拟消化结合结肠发酵技术探究BB12+GOS&FOS婴幼儿配方奶粉对肠道菌群的影响[J].中国食物与营养,2025,31(11):1-8,8.
