乳业科学与技术2025，Vol.48Issue(6)：23-29,7.DOI:10.7506/rykxyjs1671-5187-20250909-058
阿魏酸与黄原胶协同作用对β-乳球蛋白结构特征及抗原性的影响
Effects of Ferulic Acid and Xanthan Gum Interaction on Structural Characteristics and Antigenicity of β-Lactoglobulin
摘要
Abstract
The structural characteristics of proteins change when they are complexed with polyphenols and polysaccharides.This study investigated the synergistic effects of ferulic acid(FA),a dietary polyphenol,and xanthan gum(XG)at different mass concentrations on the structural characteristics and antigenicity of β-lactoglobulin(β-LG).The structural changes of β-LG after binding with FA and XG were analyzed using ultraviolet-visible(UV-Vis)absorption spectroscopy,intrinsic fluorescence spectroscopy,synchronous fluorescence spectroscopy,three-dimensional fluorescence spectroscopy,Fourier transform infrared(FTIR)spectroscopy,and molecular docking simulation.The impact of β-LG-FA-XG binding on protein antigenicity was evaluated using an indirect competitive enzyme-linked immunosorbent assay.The results demonstrated significant changes in the microenvironments around tryptophan and tyrosine residues after the complexation,along with an increase in the polarity.Furthermore,the secondary structure of the protein changed;as the mass concentration of XG increased,the relative content of β-sheets first increased and then decreased,while the relative contents of α-helices and β-turns first decreased and then increased.The relative content of random coils showed no clear trend.In addition,compared with β-LG,the β-LG-FA-XG ternary complex exhibited a significantly reduced binding capacity to immunoglobulin G(IgG),indicating decreased antigenicity.The highest IgG binding capacity inhibition rate of 17.97%was observed at an XG mass concentration of 0.10 mg/mL.关键词
β-乳球蛋白/阿魏酸/黄原胶/抗原性/相互作用Key words
β-lactoglobulin/ferulic acid/xanthan gum/allergenicity/interaction effects分类
轻工纺织引用本文复制引用
姬雅雅,赵川,梁肖娜,王佳玲,陆宇航,许一舟,毛佳怡,郑欣玥,卞熙,朱志鹏,张汶馨..阿魏酸与黄原胶协同作用对β-乳球蛋白结构特征及抗原性的影响[J].乳业科学与技术,2025,48(6):23-29,7.基金项目
大学生创新训练计划项目(202410323065Y) （202410323065Y）
