乳业科学与技术2025，Vol.48Issue(6)：36-40,5.DOI:10.7506/rykxyjs1671-5187-20250804-051
高效液相色谱法检测乳粉中免疫球蛋白G含量
Detection of Immunoglobulin G Content in Milk Powder by High Performance Liquid Chromatography
摘要
Abstract
A high performance liquid chromatography(HPLC)method for the detection of immunoglobulin G(IgG)in milk powder was established by studying the effects of immunoaffinity column performance,temperature of the sample solution during immunoaffinity column cleanup,and the type of eluent on the detection of the IgG content in milk powder.Samples were extracted with phosphate buffer solution,and the extract was purified by immunoaffinity column chromatography.The eluate was separated on a C4 reversed-phase column(250 mm×4.6 mm,3.5 μm),detected using HPLC-ultraviolet detector,and quantified by an external standard method.The results showed that the method was applicable to milk powder with different IgG contents.The standard curve exhibited good linearity in the concentration range of 10-500 μg/mL.The detection limit of the method was 80 mg/kg,and the quantification limit was 300 mg/kg.The recoveries and precision expressed as relative standard deviations(RSDs)at three spiked levels(300,600 and 3 000 mg/kg)were both aligned with the requirements of the Chinese national food safety standard GB 5009.295-2023.This method is simple,efficient and accurate,providing precise technical support for the evaluation of the nutritional value of new functional dairy products and product claims.关键词
免疫球蛋白G/乳粉/高效液相色谱法/检测Key words
immunoglobulin G/milk powder/high performance liquid chromatography/detection分类
化学化工引用本文复制引用
梁建英,唐烁,陈静,刘丽君,胡雪,吴晓莉..高效液相色谱法检测乳粉中免疫球蛋白G含量[J].乳业科学与技术,2025,48(6):36-40,5.基金项目
内蒙古自治区重点研发和成果转化计划项目(2023YFHH0093) （2023YFHH0093）
