乳业科学与技术2025，Vol.48Issue(6)：8-14,7.DOI:10.7506/rykxyjs1671-5187-20250710-045
阿魏酸与β-乳球蛋白-DHA复合物的相互作用对β-乳球蛋白致敏性的影响
Effect of the Interaction of Ferulic Acid with β-Lactoglobulin-Docosahexaenoic Acid Complex on β-Lactoglobulin Allergenicity
摘要
Abstract
β-lactoglobulin(BLG)is a main allergen in cow's milk,which can cause severe allergic reactions.This study aimed to evaluate the effect of co-modification with ferulic acid(FA)and docosahexaenoic acid(DHA)on the antigenicity and structural properties of BLG.A BLG-DHA-FA complex was obtained by non-covalent binding and its structural characteristics and antigenicity were investigated by various spectroscopic techniques,molecular docking and indirect competitive enzyme-linked immunosorbent assay.The results showed that the combination of FA and BLG-DHA changed the microenvironment surrounding aromatic amino acids,enhancing the polarity.FA and DHA bound to the hydrophobic cavity of BLG through hydrophobic interaction and hydrogen bonding,thereby changing the secondary structure of BLG.Specifically,the contents of β-sheets and random coils were reduced,and the β-turn content was increased.Compared with BLG and BLG-DHA,the addition of FA significantly reduced the antigenicity,and the highest inhibition rate against the immunoglobulin G(IgG)binding ability of 40%was obtained when the FA concentration was 0.08 mg/mL.关键词
β-乳球蛋白/二十二碳六烯酸/阿魏酸/互作效应/致敏性Key words
β-lactoglobulin/docosahexaenoic acid/ferulic acid/interaction effects/allergenicity分类
轻工纺织引用本文复制引用
杜雅宣,沙祎铖,梁肖娜,赵川,郑欣玥,毛佳怡,许一舟,陆宇航,陈代昌,赵倩,张萌..阿魏酸与β-乳球蛋白-DHA复合物的相互作用对β-乳球蛋白致敏性的影响[J].乳业科学与技术,2025,48(6):8-14,7.基金项目
大学生创新训练计划项目(X202510323030 （）
X202510323109) （）
