食品与机械2025，Vol.41Issue(11)：35-40,6.DOI:10.13652/j.spjx.1003.5788.2025.80124
超声辅助酶解—超高效液相色谱—串联质谱法测定禽蛋中叶黄素及天然VK同系物
Ultrasound assisted enzymatic hydrolysis combined with ultra-high performance liquid chromatography-tandem mass spectrometry for determination of lutein and natural vitamin K homologues in poultry eggs
摘要
Abstract
[Objective]To develop an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for determining lutein and natural vitamin K(VK)homologues,including phylloquinone(VK1),menaquinone-4(MK-4),menaquinone-7(MK-7),and menaquinone-9(MK-9),in poultry eggs by optimizing ultrasound assisted enzymatic hydrolysis.[Methods]The target compound is extracted from poultry egg samples by ultrasound assisted enzymatic hydrolysis and then separated on C18(100 mm×2.1 mm,3.0 μm)column with 0.1%formic acid aqueous solution-0.1%formic acid methanol for gradient elution.Meanwhile,the samples are quantified by external standard with APCI source for detection and analysis.[Results]Under the ultrasound assisted enzymatic hydrolysis conditions of enzyme dosage of 1 500 U and hydrolysis time of 1 hour,the linear relationships of lutein and natural VK homologues are good in the range from 1.0 ng/mL to 5 000 ng/mL(R2>0.996).The detection limit is 0.8～1.5 μg/100 g,and the quantification limit is 2.0～5.0 μg/100 g.The average recovery of lutein and natural VK homologues is 80.1%～94.9%when the spiked concentration is 10,20,and 100 μg/100 g with the RSD of 1.3%～5.5%.[Conclusion]This method has high detection efficiency,sensitivity,and selectivity and can be used as a detection method for lutein and natural VK homologues in poultry eggs.关键词
禽蛋/超声/酶解/超高效液相色谱串联质谱法/叶黄素/VK同系物Key words
poultry eggs/ultrasound/enzymatic hydrolysis/ultra-high performance liquid chromatography-tandem mass spectrometry/lutein/natural VK homologues引用本文复制引用
蔡振林,李涛..超声辅助酶解—超高效液相色谱—串联质谱法测定禽蛋中叶黄素及天然VK同系物[J].食品与机械,2025,41(11):35-40,6.基金项目
湖南省自然科学基金(编号:2022JJ90027) （编号:2022JJ90027）
