食品与发酵工业2025，Vol.51Issue(24)：9-17,9.DOI:10.13995/j.cnki.11-1802/ts.042787
组合代谢工程策略改造大肠杆菌从头合成2'-岩藻糖基乳糖
De novo synthesis of 2'-fucosyllactose through combinatorial metabolic engineering strategies in Escherichia coli
摘要
Abstract
2'-Fucosyllactose(2'-FL),the most abundant human milk oligosaccharide component in human colostrum,plays crucial roles in maintaining intestinal ecological balance,resisting the adhesion of pathogenic bacteria,regulating the immune system,and promo-ting the development of the nervous system.In this study,Escherichia coli MG1655 was employed as the host strain.First,the α1,2-fuco-syltransferase gene(futC)from Helicobacter pylori NCTC11639 was chromosomally integrated,enabling de novo 2'-FL biosynthesis using glycerol as the sole carbon source.Subsequently,a series of metabolic engineering strategies were implemented,including deletion of com-peting pathway genes(lacZ and wcaJ),overexpression of endogenous lactose permease(LacY),and enhancement of GDP-L-fucose syn-thesis pathway enzymes,thereby improving the supply of glycosyl donors and acceptors and significantly boosting 2'-FL productivity.Final-ly,replacement of wild-type FutC with the high-activity variant FutCV93I elevated 2'-FL titers to 6.57 g/L in shake flasks and 31.13 g/L in a 5 L bioreactor,providing important data support for the subsequent large-scale industrial production of 2'-FL.关键词
2'-岩藻糖基乳糖/α1,2-岩藻糖基转移酶/微生物细胞工厂/从头合成途径/生物合成Key words
2'-fucosyllactose/α1,2-fucosyltransferase/microbial cell factory/de novo synthesis/bio-synthesis引用本文复制引用
彭麟惠,于文文,邓洁莹,徐显皓,李江华,堵国成,刘龙,吕雪芹..组合代谢工程策略改造大肠杆菌从头合成2'-岩藻糖基乳糖[J].食品与发酵工业,2025,51(24):9-17,9.基金项目
国家自然科学基金项目(32400054) （32400054）
