沈阳农业大学学报2025，Vol.56Issue(6)：103-112,10.DOI:10.3969/j.issn.1000-1700.2025.06.010
大豆细胞质雄性不育系及其保持系花器官生理及转录组差异分析
Physiological and Transcriptomic Differences in Floral Organs between Cytoplasmic Male Sterile Lines and Their Maintainers in Soybean
摘要
Abstract
[Objective]Study was carried out to investigate the physiological and transcriptome differences of flower organs in soybean cytoplasmic male sterile lines and their homologous maintainer lines,fully explore the fertility genes of maintainer lines,cultivate stronger maintainer lines,and improve hybrid seed production yield.[Methods]Using cytoplasmic male sterile line JLCMS116A and its homologous maintainer line JLCMS116B as materials,the carbohydrate,soluble protein,endogenous hormones,and transcriptome of flower buds during their peak flowering period were analyzed and studied.[Results]Analysis of the five major hormones in the flower buds of sterile and maintainer lines showed that auxin and cytokinin in the flower buds of sterile lines were lower than those of their homologous maintainer lines,while ethylene content was higher than that of their homologous maintainer lines;the analysis of soluble sugar,soluble protein,and starch content in the flower buds of sterile and maintainer lines showed that the content of soluble sugar,starch,and soluble protein was lower than that of their homologous maintainer lines;the transcriptome sequencing results showed that a total of 473 differentially expressed genes were obtained,of which 426 were upregulated and 11 were downregulated.The differentially expressed genes were significantly enriched in metabolic pathways such as pollen tube growth,cell wall modification,starch and sucrose metabolism,protein binding,protein serine/threonine kinase activity,biosynthesis of other secondary metabolites,fructose and mannose metabolism,galactose metabolism,inositol phosphate metabolism,and interconversion of pentose and glucuronic acid.Among them,11 down regulated genes were mainly annotated for ascorbic acid and aldehyde metabolism,amino sugar and nucleotide sugar metabolism,base excision repair metabolic pathways and nucleotide sugar metabolism processes,pollen maturation to photoperiod flowering,metal ion transport,and redox reaction GO enrichment classification.[Conclusions]Glyma.11G036600 may be a key gene affecting pollen fertility differences between cytoplasmic male sterile line JLCMS116A and its homologous maintainer line JLCMS116B.关键词
大豆/不育系/保持系/转录组Key words
soybean/sterile line/maintainer line/transcriptome分类
农业科技引用本文复制引用
何德鑫,李志刚,王晓东,毕文波,胡浩,陈新,刘鹏,丛百明,孙佳尧,张鹏宇..大豆细胞质雄性不育系及其保持系花器官生理及转录组差异分析[J].沈阳农业大学学报,2025,56(6):103-112,10.基金项目
内蒙古自治区"揭榜挂帅"项目(2023JBGS0006) （2023JBGS0006）
通辽地区大豆高产栽培技术研究项目(MDK2023102) （MDK2023102）
