食品科学技术学报2025，Vol.43Issue(6)：107-121,15.DOI:10.12301/spxb202500218
基于转录组学的低磷胁迫激活白黄链霉菌TD-1高效合成帕马霉素机制研究
Study on Mechanism of Low-Phosphate Stress Activated Efficient Pamamycin Biosynthesis in Streptomyces alboflavus TD-1 Based on Transcriptomic Analysis
摘要
Abstract
Pamamycin,produced by Streptomyces alboflavus TD-1,showed good application prospects in the prevention and treatment of Aspergillus flavus due to its significant biological activity.In order to break through the bottleneck of low pamamycin production,the molecular regulation mechanism of low-phosphate conditions to promote the efficient synthesis of pamamycin was analyzed by transcriptome sequencing and RT-qPCR technology with Streptomyces alboflavus TD-1 as the research object.The results showed that compared with the control group,the low phosphorus condition significantly increased the level of pamamycin synthesis by Streptomyces alboflavus TD-1(2.01-fold increase in pamamycin production at 120 μmol/L phosphate concentration).Transcriptome analysis showed that 655 differentially expressed genes(DEGs)were detected after 48 h of culture under low phosphorus conditions.At 96 h,the number of DEGs increased to 9 802,including 1 777 up-regulated genes and 8 025 down-regulated genes.GO and KEGG analyses showed that DEGs were significantly enriched in GO terms such as translation and primary metabolic processes,as well as KEGG pathways such as ribosome synthesis and TCA cycle.Metabolic pathway analysis further found that low phosphorus stress activated the expression of genes in the pamamycin synthesis pathway genes,the central carbon metabolism pathway genes,and the SenX3-RegX3 two-component system genes.At the same time,it inhibited the expression of genes in the precursor competitive pathway of pamamycin,such as biotin synthesis genes(bioA,bioB)and fatty acid synthesis genes(fabF,fabH).The results showed that under low phosphorus conditions,Streptomyces albus TD-1 sensed phosphorus signals through the SenX3-RegX3 two-component system,activated central carbon metabolism and inhibited fatty acid synthesis,so as to accumulate the precursor of pamamycin synthesis and activate the expression of pamamycin synthesis genes to promote its efficient synthesis.The aim of this study was to provide a basis for the efficient biosynthesis of pamamycin and promote its application in the biological control of Aspergillus flavus.关键词
白黄链霉菌/低磷胁迫/帕马霉素/转录组学分析/次级代谢产物Key words
Streptomyces alboflavus/low phosphate stress/pamamycin/transcriptomic analysis/secondary metabolites分类
轻工纺织引用本文复制引用
DONG Zehua,ZHAO Hailei,LI Wangqiang,GUO Qingbin,DING Wentao,WANG Changlu..基于转录组学的低磷胁迫激活白黄链霉菌TD-1高效合成帕马霉素机制研究[J].食品科学技术学报,2025,43(6):107-121,15.基金项目
国家自然科学基金资助项目(31972177).National Natural Science Foundation of China(31972177). （31972177）
